氨基胍对内毒素血症山羊肝线粒体自由基代谢的影响  

Effects of aminoguanidine on the metabolism of free radicals of liver mitochondria in goats with endotoxemia

作　　者：刘玉清[1] 郭剑英[1] 刘静[1] 梁群超[1] 唐兆新[1] 

机构地区：[1]华南农业大学兽医学院

出　　处：《中国兽医杂志》2008年第10期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金资助项目(30371068)

摘　　要：为探讨氨基胍对内毒素血症山羊肝线粒体自由基代谢的影响,将48只健康山羊随机分为4组,生理盐水(SL)组、内毒素(LPS)组、氨基胍(AG)组和氨基胍(AG+LPS)保护组。每组分别取肝组织,提取肝线粒体,检测T-SOD、T-AOC、CAT、GSH-Px和GR活性及MDA含量的变化。结果显示,内毒素血症时山羊肝线粒体中SOD、GSH-Px、GR和CAT活性降低,T-AOC活力下降,MDA含量明显增加,而氨基胍保护组肝线粒体抗氧化酶活性普遍回升,MDA含量明显下降。提示,氨基胍能减轻内毒素血症山羊因脂质过氧化造成的肝线粒体损伤,保护肝线粒体功能。To explore effects of aminoguanidine on the metabolism of free radicals of liver mitochondria in goats with endotoxemia, 48 normal goats were divided randomly into four groups that were respectivelytreated with saline（SL） ,LPS,AG and AG＋LPS,liver mitochondria was determined for evaluation of con- centrations of T-SOD.T-AOC,CAT.GSH-PX,GR and MDA. Results showed that MDA content of LPS group increased gradually, the activities of mitochondrial enzymes decreased apparently, but the activities of all antioxidases of AG＋ LPS group improved much better than that of LPS group, MDA content of AG ＋LPS group decreased apparently, compared to LPS group. The results indicated that aminoguanidine could reduce lipid peroxidation injury of liver mitochondria and could protect liver mitochondrial functions in goats with endotoxemia.

关 键 词：氨基胍 山羊 内毒素血症 肝线粒体 抗氧化酶 脂质过氧化 

分 类 号：S852.23[农业科学—基础兽医学]