氧化亚铁硫杆菌铜代谢基因Afe0329的转录子鉴定及分析  

Identification and Analysis of Afe0329 Transcripton Relative to Copper Homeostasis in Acidithiobacillus ferrooxidans

作　　者：罗焱杰[1,2] 廖荷欢[1,2] 张成桂[1,2] 管昊[1,2] 罗海浪[1,2] 刘学端[1,2] 邱冠周[1,2] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]中南大学生物冶金教育部重点实验室,长沙410083

出　　处：《微生物学通报》2008年第10期1538-1543,共6页Microbiology China

基　　金：国家"973项目"(No.2004CB619201);国家自然科学基金项目(No.50621063;No.30428014)

摘　　要：采用reverse transcriptase PCR技术,研究了嗜酸氧化亚铁硫杆菌(Acidithiobacillus fer-rooxidans)标准菌株ATCC23270中铜代谢基因Afe0329的转录子情况;用Vector NTI、Blast、TMHMM Server和PSORTb等软件对此转录子中的各基因做了进一步的生物信息学分析;并用此转录子的引物扩增多种细菌DNA,以鉴定此转录元件在A.ferrooxidans菌株基因组中的普遍性。结果表明基因Afe0329,Afe0330和Afe0331一起转录,处于同一个转录子;基因Afe0329为编码定位于膜上P1b3型ATP酶基因,Afe0330为编码定位于细胞质中的蛋白基因,Afe0330和Afe0331的Blast分析都没有意义的同源性序列;且此转录元件普遍存在于A.ferrooxidans菌株基因组中。With reverse transcriptase PCR, the transcripton of copper homeostasis relative gene Afe0329 in Acidithiobacillus ferrooxians standard strain ATCC23270 was investigated. The further analysis of genes in this transcripton was analyzed employed by Vector NTI, Blast, TMHMM Server, PSORTb software and so on. From the DNA of different strains, the transcripton of Afe0329 was amplified using special primer pairs to identify the universality of it in the genome of A.ferrooxidans strains. The results showed that gene Afe0330 and Afe0331 were cotranscribed with Afe0329, and they were in a single transcripton. Gene Afe0329 was supported to express a P1 b3-type ATPase which is a heavy metal ion pumping transmembrane protein, protein AFE0330 which expressed by gene Afe0330 was a cytoplasmic protein, no significant homologous sequences of Afe0330 or Afe0331 had been obtained by Blast analysis. And the transcripton of Afe0329 was universal in genome of A. ferrooxidans strains.

关 键 词：氧化亚铁硫杆菌 P型ATP酶 铜代谢 转录子 Reverse transcriptase PCR 

分 类 号：Q93[生物学—微生物学]