HPLC法测定刺囊毛霉微生物转化甘草次酸的主产物含量  

Determination of 7β-hydroxyglycyrrhetinic Acid in Microbial Transformation of Glycyrrhetinic Acid by Mucor spinosus by HPLC

作　　者：马晶[1] 辛秀兰[1,2] 袁其朋[1] 吴志明[2] 李晓燕[2] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]北京电子科技职业学院生物技术应用中心,北京100029

出　　处：《微生物学通报》2008年第10期1664-1667,共4页Microbiology China

基　　金：北京市属市管高等学校人才强教计划

摘　　要：采用刺囊毛霉AS3.3450对甘草次酸进行微生物转化,生成的主产物经分析鉴定是7β-羟基甘草次酸。采用高效液相色谱方法,以C18-ODS为色谱柱,甲醇-0.03%三氟乙酸水溶液(梯度洗脱)为流动相,检测波长为:254nm,测得在160r/min、27°C的转化条件下,底物加量为130mg/L,转化时间为9d时,主产物得率最高为712mg/g甘草次酸,且在选定色谱条件下7β-羟基甘草次酸的线性范围良好,平均加样回收率为98.1%,平均标准偏差(RSD)为2.37%。The major product of microbial transformation of glycyrrhetinic acid by Mucor spinosus AS3.3450 has been identified as 7β-hydroxyglycyrrhetinic acid on the basis of its spectral data. 7β-hydroxyglycyrrhetinic acid was directly determined by HPLC. Methanol and ultra pure water containing 0.03% trifluoroacetic acid was used as the mobile phase on C18 column, the UV detection wavelength was 254 nm. The maximum transformation rate observed was 712 mg 7β-hydroxyglycyrrhetinic acid/g glycyrrhetinic acid on day 9 under 27℃, 160 r/min and substrate at a concentration of 130 mg/L. Results show that the content of 7β-hydroxyglycyrrhetinic acid indicates a good linearity under these conditions with a average recovery of 98.1% and a RSD of 2.37%.

关 键 词：高效液相色谱 微生物转化 甘草次酸 7β-羟基甘草次酸 

分 类 号：R284[医药卫生—中药学]