雷公藤多甙对大鼠慢性非细菌性前列腺炎NF-κB及iNOS表达影响的实验研究  

The Influence of Tripterygium wilfodil polyglucosidinm(TWP) on the expression of NF-κB and iNOS in the rat model of nonbacterial chronic prostatitis

作　　者：杨镒魟[1] 张唯力[2] 梁勇[1] 刘建辉[1] 

机构地区：[1]四川省自贡市第四人民医院泌尿外科,自贡643000 [2]重庆医科大学附属第二医院泌尿外科

出　　处：《中国男科学杂志》2008年第9期14-18,共5页Chinese Journal of Andrology

基　　金：重庆市卫生局医学科研项目(07-2-100)资助

摘　　要：目的探讨雷公藤多甙对慢性非细菌性前列腺炎大鼠前列腺组织NF-κB和iNOS表达的影响。方法将30只动物随机分为3组:正常对照组、慢性非细菌性前列腺炎模型组(简称前列腺炎模型组)和慢性非细菌性前列腺炎+雷公藤多甙治疗组(科称雷公藤治疗组),每组10只,分别检测前列腺组织中白细胞总数和卵磷脂小体密度变化以及NF-κB和iNOS在各组大鼠前列腺中的表达情况。结果(1)雷公藤治疗组与正常对照组相比,前列腺炎模型组前列腺内白细胞数明显增多(P<0.01),卵磷脂小体密度降低(P<0.05),NF-κB和iNOS表达强度显著高于正常组(P<0.01);(2)雷公藤治疗组与前列腺炎模型组相比,雷公藤治疗组前列腺内白细胞数明显减少(P<0.01),卵磷脂小体密度增加(P<0.05),NF-κB和iNOS蛋白表达与前列腺炎模型组表达有显著差异(P<0.01)。结论NF-κB和iNOS可能参与了慢性非细菌性前列腺炎发病过程,雷公藤多甙可以使慢性非细菌性前列腺炎的炎症明显好转。Objective To explore the influence of tripterygium polyglucoside on the expression of NF-κB and iNOS in the rat model of nonbacterial chronic prostatitis. Methods Thirty aged SpragueDawley rats were randomly divided into three groups： the control group（10 rats）, the untreated group（rat model of nonbacterial chronic prostatitis, 10 rats）and the treated group （rat model of nonbacterial chronic prostatitis treated with TWP group, 10 rats）.The amount of WBC and lecithin body in prostate tissue were measured and the expressions of NF-κB and iNOS mRNA in rat Prostate tissue were detected. Results （1） Compared with that in the control group, the amount of WBC increased untreated group （P〈0.01） and the density of lecithin body decreased（P〈0.05）. The mRNA expressions of NF-κB and iNOS were upregulated in untreated group in comparision with that in control group （P〈0.01）; （2）Compared with that in untreated group, the amount of WBC decreasedin treated group （P〈0.01） and the density of the lecithin body increased （P〈0.05）. There were obvious differences in the mRNA expressions of NF-κB and iNOS between the treated goup and the untreated group（P〈0.01）. Conclusion The iNOS and NF-κB might be involved in the development of the nonbacterial chronic prostatitis. TWP could be used for the treatment of nonbacterial chronic prostatitis.

关 键 词：核因子-ΚB 诱导型一氧化氮合酶 雷公藤多甙 前列腺炎 

分 类 号：R697.33[医药卫生—泌尿科学]