包合铁卟啉的环糊精聚合物作为过氧化物朊酶模型物的研究  

Study on Immobilized Supramolecular inclusion Complex of Iron-Porphyrin as an Analogue for Peroxide Proteinase

作　　者：毛陆原[1] 朱敏[1] 黄雪梅[1] 沈含熙[1] 李荣[2] 

机构地区：[1]南开大学化学系,天津300071 [2]天津商学院

出　　处：《高等学校化学学报》1997年第10期1611-1615,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：将β-环糊精交联聚合物（β-CDP）包合铁卟啉（FeTPPS4）形成的固相超分子作为过氧化物朊酶的模拟物，考察影响超分子包合物形成的各种因素，研究了固相超分子催化苯酚一过氧化氢的反应机理，测定了固相超分子的稳定常数，探讨了各种底物对固相超分子酶的模拟物的影响．实现了过氧化氢对4-氨基安替比林一对氯苯酚的催化显色反应，并应用于过氧化氢的酶法分析．The Supramolecular inclusion complex of iron-5, 10, 15, 20-tetrakis[4-sulfophenyl]-21H, 23H-porphin (FeTPPS4 ) with p-cyclodextrin cross-linking polymer(β-CDP) was obtained and used as an analogue for peroxide proteinase. The effects of variedfactors on formation constant of the inclusion complex were investigated. The mechanism ofimmobilized supramolecular complex catalyzing p-chlorophnic acid by hydrogen peroxide wasstudied in detail. The immobilized inclusion complex as mimests of horseradish peroxidasewas very stable and found to exhibit the high proteinase-like activity, which have beendemonstrated by enzymatic methods of analysis. The method was applied to determinehydrogen peroxide with 4-aminoantipyrine and p-chlorophenic acid. The calibration curve forabsorbance-concentration(H2O2) was linear from O to 3. 0 μg/mL and the linear correlationcoefficient was 0. 999 4.

关 键 词：过氧化氢 酶法分析 铁卟啉 Β-CD 模拟酶 

分 类 号：O657.303[理学—分析化学] O613.3[理学—化学]