习惯性流产的抗凝血酶原抗体IgG、IgM检测的初步应用  

Detection of Antiprothrombin Antibodies IgG,IgM in Patients with Recurrent Abortion

作　　者：林青[1] 彭维林[2] 李庶甘[1] 唐文吟[2] 张毅[1] 赵越真[2] 张荣盛[1] 吕华北[2] 

机构地区：[1]厦门出入境检验检疫局国际旅行保键中心实验室,厦门361012 [2]福建泉州市儿童医院/妇幼保健院,泉州362000

出　　处：《血栓与止血学》2008年第5期218-220,共3页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的建立人血浆抗凝血酶原抗体(aPT)IgG、IgM的间接ELISA检测方法,并用于检测原因不明习惯性流产病人。方法采用人凝血酶原包被微孔板,以碱性磷酸酶标记的抗人IgG、IgM为检测抗体,对35例原因不明习惯性流产患者血浆中的抗凝血酶原抗体IgG、IgM、IgG+IgM进行检测,同时计算其阳性率和优势比(OR,95%可信限)。结果所建方法线性范围广,稳定性、特异性、重复性较好。原因不明习惯性流产患者血清中抗凝血酶原抗体IgG、IgM、IgG+IgM阳性率分别为14.3%、11.4%和20%,对照组分别为2.5%、2.5%和5%,P值分别为0.073、0.14及0.05。结论该法是一种较好的抗凝血酶原抗体IgG、IgM定量测定法。原因不明习惯性流产的aPT较高于正常妇女,可以作为原因不明习惯性流产患者的辅助诊断指标之一。Objective To establish an indirect solid phase ELISA method for the detection of antipro- thrombin （aPT）IgG and IgM, and apply in patients with unknown recurrent abortion. Methods A indirect ELISA using the purified human prothrombin for plate coating and AKP-labeled goat anti-human IgG or IgM as detection antibody was applied to quantify aPT IgG or IgM levels in plasmas from 35 patients with habitud aboreion （HA）. Their positive rates and odds ratio （ OR ： with 95 % confidence interval, CI） were computed. Re- suits This method had high precision and good linearity and show good sensitivity, specificity, reproducibility and stabilization. The positive rates of IgG, IgM, IgG ＋ IgM- aPT were 14.3%, 11.4% and 20% , respectively, with unknown recurrent pregnancy loss,compared with 2.5% ,2.5% and 5% in the controls,P value were 0.073,0.14 and 0.05. Conclusion aPT are more frequently found in patients with HA than in controls. The anti prothrombin assay was validated for the detection of aPT, IgG, IgM, in patients .

关 键 词：抗凝血酶原抗体 抗磷脂抗体 流产 

分 类 号：R392[医药卫生—免疫学]