低叶绿素b水稻突变体的抗氧化酶系统研究  

Antioxidase System of a Rice Mutant With Low Chlorophyll b

作　　者：谢雅晶[1] 陈海燕[1] 张荣铣[2] 张炜[1] 

机构地区：[1]南京农业大学生命科学学院生物化学与分子生物学系,江苏南京210095 [2]南京农业大学生命科学学院植物科学系,江苏南京210095

出　　处：《安徽农业科学》2008年第27期11650-11652,11657,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30400030);江苏省创新人才基金资助项目

摘　　要：[目的]研究低叶绿素b水稻突变体抗氧化酶系统在缓解光氧化伤害中的作用。[方法]以低叶绿素b水稻突变体和野生型为材料,对其叶绿体中过氧化氢(H2O2)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和同工酶谱进行了比较。[结果]与野生型相比,突变体叶片细胞及叶绿体中抗氧化酶SOD、POD和CAT的同工酶种类相对较多,酶活性相对较高;强光条件下,突变体叶绿体H2O2的含量低于野生型叶绿体。[结论]较强的内源活性氧清除系统减轻了强光下过剩光能对光合膜的光氧化伤害,是该突变体PSII具有较高稳定性的重要原因。[Objective]The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] The rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparative research their peroxide（H2O2） content,the activity and isozyme of superoxide dismutase（SOD）,catalase（CAT） and peroxidase（POD） in chloroplast.[Result] Compared with the wild type,there were many kinds of SOD,POD and CAT isozyme in leaf cells and chloroplast of mutant,and the enzyme activity of SOD,POD and CAT isozyme in leaf cells and chloroplast of mutant was also higher correspondingly.Under intense light condition,the H2O2 content of chloroplast in mutant was less than that in the wild type.[Conclusion]The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane,which is an important reason for higher stability of this mutant PSII.

关 键 词：低叶绿素b含量突变体 水稻(OryzasativaL.) 抗氧化酶 同工酶谱 

分 类 号：S511[农业科学—作物学]