^(60)Co-γ射线诱变猕猴桃枝条变异的SSR研究  

Study on SSR of Mutation of Kiwifruit Branches Induced by ^(60)Co-γ Radiation

作　　者：陈树丰[1] 刘文[1] 杨妙贤[1] 梁红[1] 

机构地区：[1]仲恺农业工程学院生命科学学院,广东广州510225

出　　处：《安徽农业科学》2008年第27期11679-11681,11687,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省科技攻关项目(C20617)

摘　　要：[目的]为猕猴桃的辐射诱变育种提供借鉴。[方法]用25、50和75 Gy 3个剂量的60Co-γ射线处理2个品种猕猴桃不同植株的枝条,提取其嫩叶基因组DNA,并进行不同枝条嫩叶DNA之间的SSR多态性分析,从分子水平上分析辐射诱变效应。[结果]2个品种的SSR多态性变化表现出一定的差异性。不同剂量辐射处理后枝条的成活率有所差异;辐射处理后2个品种猕猴桃的SSR扩增带明显增加,且随着辐射剂量的增加而越加明显,表现为经50 Gy60Co-γ处理的枝条的SSR扩增带多于25 Gy处理者。"和平红阳"猕猴桃SSR扩增带数目高于"和平1号"猕猴桃。[结论]该研究为猕猴桃辐射诱变育种的分子机理研究奠定了基础。[Objective] The aim was to provide the reference for the irradiation breeding of kiwifruit.[Method] 60^Co-γ ray with 3 doses of 25,50 and 75 Gy were used to radiate the branches of different plants of 2 kiwifruit varieties,the genomic DNA of the tender leaves were extracted and the mutation effects after radiation were analyzed at the molecular level through analyzing the SSR polymorphism among the tender leaf DNA in different branches.[Result] The SSR polymorphism variation of the 2 varieties showed certain difference.The survival rate of the branches was different after they were treated by different doses of radiation.The SSR amplified band of the 2 kiwifruit varieties was increased obviously after radiation and the band was more obvious with the increase of the radiation dose,which exhibited that the SSR amplified band of the branches treated by 60Co-γ at 50 Gy was more than that treated by 60Coγ at 25 Gy.The SSR amplified bands of ＇Heping hongyang＇ Actinidia chinensis were more than that of ＇Heping No.1＇Actinidia deliciosa.[Conclusion]The research laid the foundation for the study of the molecular mechanism for the irradiation breeding of kiwifruit.

关 键 词：猕猴桃 辐射 SSR 

分 类 号：S653.4[农业科学—果树学]