空肠弯曲菌热休克蛋白43对T细胞的活化作用  

HSP43 of campylobacter jejuni induces T cells activation

作　　者：臧星星[1] 马宝骊[1] 柏峻[1] 王利[1] 高建新[1] 张汉明[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所

出　　处：《中国病理生理杂志》1997年第5期461-464,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金;卫生部及上海市高教局资助

摘　　要：本文用３Ｈ－ＴｄＲ掺入试验发现空肠弯曲菌４３ＫＤ热休克蛋白（ＨＳＰ４３）能显著促使小鼠脾淋巴细胞活化，并呈剂量依赖关系。这种活化作用不但能被特异性针对ＨＳＰ４３的二株单抗明显抑制，而且也能被针对ＨＳＰ６０家族保守区序列的二株单抗所抑制。去除巨噬细胞同样也能抑制ＨＳＰ４３活化作用。用单抗和补体的特异性杀伤所致Ｔ细胞亚群缺失试验发现ＨＳＰ４３主要活化ＣＤ４＋Ｔ细胞。本文结果提示空肠弯曲菌ＨＳＰ４３可能具有某些超抗原特性，这对解释ＨＳＰ诱导自身免疫病具有一定意义。In the experiment of murine splenic lymphocytes transformation,43KD heat shock protein(HSP43)of campylobacter jejuni,strain CJ-S131,was found to be capable of polyclonal activation.Uptake of3H-TdR transformation was linearly correlated with dosage of HSP43.Monoclonal antibodies IIH9,ML-30,CJ-1 and CJ-2 specific for widely separated linear amino acid epitopes of HSP43 strongly inhibited the activation of HSP43 on T cells,percentage of inhibition from 30% to 79% when 1:100 dilution of antibodies added into the culture.The activation of T cells by HSP43 was also strongly inhibited by removal of macrophages from lymphocytes population.Depletion of lymphocyte subset with monoclonal antibodies against CD3,CD4,CD8 and complement showed that lymphocyte subset activated by HSP43 was CD4+T cells.These results suggested that HSP43 of campylobacter jejuni might have some features of a superantigen,and also gave us an insight into the role of heat shock proteins in autoimmune diseases.

关 键 词：弯曲杆菌属 热休克蛋白 T细胞 免疫学 

分 类 号：R392.12[医药卫生—免疫学]