重组质粒DNA-抗原-抗体复合物诱生乙型肝炎表面抗体的研究  

Humoral immune response enhanced by immunization with anti-HBs and HBsAg complex plus DNA vaccine

作　　者：瞿涤[1] 闻玉梅[1] 周生华[1] 王开利 余生玲 郭盛淇 李平洋[1] 

机构地区：[1]上海医科大学卫生部医学分子病毒学重点实验室,200032

出　　处：《上海医学》1997年第12期699-701,共3页Shanghai Medical Journal

基　　金：国家863高科技计划资助!102-07-01-01

摘　　要：本文研究了含编码乙型肝炎病毒表面抗原（HBsAg）基因的重组质粒DNA（pCMV－HBS）对HBsAg、抗·HBS免疫复合物（IC）诱生抗-HBs的影响。在Balb／c小鼠中，IC加PCMV－HBS（100μg）免疫组比单独使用IC或pCMV－HBsDNA组诱生的抗-HBs效价高，虽然略低于IC加氢氧化铝组，但无明显差异。在IC中加20μgpCMV-HBsDNA。经再次免疫亦可有效地诱生高效价的抗-HBs，可达PCMV－HBSAg100μg加IC免疫一次所诱生的杭体水平。然而在IC中加入1μgpCMV－HBs，即使再次免疫也不能使小鼠抗-HBS的阳转率达100％。此外在IC中加入载体质粒DNA（pCMV），亦可增强诱生抗-HBS。当DNA用量为100μg或20μg时，pCMV－HBs加入组诱生的抗-HBs效价略高于加入相同量的载体质粒DNA组。结果提示可用HBsAg-抗HBs免疫原性复合物加重组质粒DNA组建治疗性疫苗。Previous studies showed that anti-HBs and HBsAg immunogenic complex (IC) could induce higher hu- moral and cellutar wine response than only HBsAg. The plasmid DNA encoding HBsAg gene could also stimulate an- ti-HBs immune response, especially strong CTL activity. In the present study, we immunized Balb/c mice with IC plus DNA encoding HBsAg(pCMV-HBs) to study whether they could stimulate stronger immune response than conventional vaccines. Eariler and higher titer anti-HBs antibody was induced by IC plus pCMV-HBs(100μg per mouse)than by IC or pCMV-HBs alone. Less pCMV-HBs (20μg or lμg per mouse) added into IC could also enhance anti-HBs response. Af- ter boosting, the titer of anti-HBs induced by IC plus 20μg of pCMV-HBs could reach the level immunized with IC plus 100μg of pCMV-HBs, while IC plus 1μg of pCMV-HBs could not induce 1OO% anti-HBs sero-conversion in mice. Inter- estingly, the plasmid vector (pCMV) added into IC could also enhance anti-HBs immune response. At the dosages of 100μg and 20μg of DNA, the titer of anti-HBs induced by pCMV-HBs plus IC was slightly higher than that by pCMV plus IC. The resuIts showed that pCMV-HBs or pCMV DNA could improve immune response induced by IC of HBsAg- anti-HBs.

关 键 词：免疫原性复合物 DNA免疫 乙型肝炎 

分 类 号：R512.620.3[医药卫生—内科学]