猪圆环病毒2型河北株的全基因组克隆与系统进化分析  

Cloning and Evolution Relationship Analysis of Complete Genomes of Type 2 Porcine circovirus Isolated from Hebei Province

作　　者：王光祥[1] 郑海学[1] 刘湘涛[1] 尚佑军[1] 常艳燕[1] 靳野[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《动物医学进展》2008年第10期1-4,共4页Progress In Veterinary Medicine

基　　金：国家重点基础研究发展计划(973)项目(2005CB523201)

摘　　要：应用PCR方法从疑似断奶仔猪多系统衰竭综合征(PMWS)的死亡仔猪组织病料中扩增出PCV-2全基因组(1767bp),将此基因片段克隆入pMD20-T载体,筛选获得重组质粒pMD-PCV2并对其进行序列测定,然后对全基因组进行同源性和遗传进化分析。结果表明,PCV-2分离毒株与参考株的全基因组同源性介于94%～98.4%之间,ORF1的同源性介于97.0%～98.8%,ORF2的同源性略低,介于91.9%～98.4%之间。该毒株与荷兰株、广西株和上海株等在一个进化分支上。本研究有助于监测PCV-2的疫源和进化关系,为进一步深入研究病毒基因功能奠定一定基础。The full-length nucleotide sequence of PCV-2 was amplified by PCR from the tissues of clinical pigs with postweaning multisystemie wasting syndrome. The fragment amplified was cloned into pMD 20- T vector and the recombinant plasmid was sequenced and determined. Then, the homology and evolutional relationship of PCV-2 genome and reference strain sequences were analyzed. The results of homology analysis showed that genome of Hebei isolate shared 94.0 %-98.4 % homology with reference sequence and the ORF1 exhibited 97. 0%-98. 8% sequence similarity, but the ORF2 showed lower homology, 91. 9%- 98.4%. Phylogenetic analysis revealed that the Hebei strain is clustered in the PCV-2. The study would play a foundation to understand the genic function of the virus.

关 键 词：猪圆环病毒2型 基因组 克隆 序列分析 

分 类 号：S852.659.2[农业科学—基础兽医学] S858.28[农业科学—兽医学]