HPLC测定中肺合剂中防己诺林碱和粉防己碱的含量  

Determination of Fangchinoline and Tetraridrine in Zhongfei Mixture by HPLC

作　　者：林能明[1,2] 张如松[2] 方罗[1] 孔维月[2] 

机构地区：[1]浙江中医药大学附属浙江省肿瘤医院,杭州310022 [2]浙江中医药大学药学院,杭州310053

出　　处：《中国药学杂志》2008年第20期1593-1595,共3页Chinese Pharmaceutical Journal

基　　金：浙江省中医药科技计划(2007CA098)

摘　　要：目的测定中肺合剂中防己诺林碱、粉防己碱的含量。方法样品经预处理后采用RP-HPLC同时测定它们的含量。色谱条件：色谱柱为Agilent Zorbax Elipse XDB—C18；流动相为0．06％三乙胺-乙腈溶液（44：56）；流速为1．0mL·min^-1；检测波长设在240nm；柱温30℃。结果防已诺林碱在0．4375—17．5mg·L^-1与粉防己碱在0．875—35．0mg·L^-1内，峰面积与其浓度具有良好的线性关系。平均加样回收率分别为89．30％～94．35％（防己诺林碱），90．08％-95．74％（粉防己碱）。结论本方法简便、快速、准确，可同时测定中肺合剂中防己诺林碱和粉防己碱的含量。OBJECTIVE To establish a method for the determination of fangehinoline and tetraridrine in Zhongfei mixture. METHODS The separation was performed on an Agilcnt Zorbax Elipse XDB-C18 column at 30 ℃ and detected at 240 nm , the mobile phase consisted of 0. 06% triethylamine and aeetonitrile （ 44 ： 56 ） at a flow rate of 1.0 mL·min^-1 . RESULTS The calibration curves of fangchinoline and tetraridrine had excellent linearity between the range of 0. 437 5 -17. 5 mg·L^-1 and 0. 875 - 35.0 mg·L^-1. The average recoveries of spiked with sample were 89. 30% -94. 35% for fangchinoline and 90. 08% -95.74% for tetraridrine. CONCLUSION The method is sensitive, convenient and accurate, and is suitable for the simultaneous determination of fangchinoline and tetraridrine in Zhongfei mixture.

关 键 词：中肺合剂 防己诺林碱 粉防己碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]