斑点免疫印迹法快速测定水蛭炮制品中水蛭素水解产物含量  

Rapid determination of contents of hirudin's hydrolysates in processed leech by dot blotting

作　　者：王厚伟[1] 田景振[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《中国中药杂志》2008年第19期2193-2195,共3页China Journal of Chinese Materia Medica

基　　金：山东省教育厅(J06L17);山东省优秀中青年科学家奖励基金(2007BSB14087);山东省中药理论与技术创新团队资助

摘　　要：目的:测定4批次水蛭炮制品水蛭素水解产物含量,建立斑点免疫印迹法评价相应生药材药效成分明确的中药炮制品质量新方法。方法:以鼠抗水蛭素单抗为一抗,应用斑点免疫印迹法对水蛭炮制品中水蛭素水解产物作定性检测,杂交信号应用Quantity one软件作定量分析。结果:4批次水蛭炮制品所含水蛭素水解产物的质量分数分别为296.51,165.47,95.58,298.05μg.g-1。结论:不同批次水蛭炮制品之间水蛭素水解产物含量差异显著,斑点免疫印迹法简便快捷,结果准确,无需大型设备,可用于水蛭与类似中药材炮制品的品质评价。Objective： To determine the contents of hirudin＇s hydrolysates in processed leeches, set up a new evaluating method of dot blotting to evaluate the qualities of those processed Chinese medicines as leeches. Method： Contents of hirudin＇s hydrolysates in processed leeches were determined by dot blotting with rat antibody of anti-hirudin as the first antibody. Blotting signal was analyzed by software of Quantity One. Result： Contents of hirudin＇s hydrolysates in four batches of processed leeches were 296. 51, 165.47, 95.58, and 298.05μg·g^- 1, respectively. Conclusion： Difference among four batches of processed leeches was significant in the content of hirudin＇s hydrolysates. Dot blotting, as a convenient and accurate method can be broadly used for evaluating processed products of Chinese crude drugs similar to leeches.

关 键 词：水蛭 斑点印迹 水蛭素 

分 类 号：R284.1[医药卫生—中药学]