RP-HPLC同时测定淡竹叶中4种黄酮苷的含量  

Simultaneous determination of four glycosylflavones from Lophatherum gracile by RP-HPLC

作　　者：袁珂[1] 薛月芹[1] 殷明文[2] 楼炉焕[1] 

机构地区：[1]浙江林学院天然药物研发中心,浙江临安311300 [2]郑州大学化学系,河南郑州450052

出　　处：《中国中药杂志》2008年第19期2215-2218,共4页China Journal of Chinese Materia Medica

基　　金：浙江省森林培育重中之重学科开放基金项目(200510)

摘　　要：目的:建立RP-HPLC同时测定不同产地、不同采收期淡竹叶中荭草苷、异荭草苷、牡荆苷、异牡荆苷的含量。方法:采用高效液相色谱法,Waters XBridge C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.05%冰醋酸(35∶65)为流动相洗脱,流速1.0 mL.min-1,检测波长340 nm,柱温25℃。结果:4种成分均达到基线分离,线性关系良好。方法的平均回收率及RSD分别为荭草苷103.2%,2.1%;异荭草苷101.6%,2.7%;牡荆苷98.4%,2.3%;异牡荆苷99.2%,1.8%。结论:本研究所建立的HPLC方法操作简便、结果准确可靠,为该类药材的入药及资源利用提供了依据。Objective： To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time. Method： The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column （4. 6 mm×250 mm, 5μm）. The mobile phase consisted of methanol- 0.05% acetic acid （35： 65）. The flow rate was 1.0 mL·min^-1 and the detection wavelength was set at 340 nm. The column temperature was set at 25℃. Result： Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2. 1% ; 101.6%, 2. 7% ; 98.4%, 2. 3% ; 99.2% , 1.8%, respectively. Conclusion： The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.

关 键 词：RP-HPLC 淡竹叶 黄酮苷 

分 类 号：R284.1[医药卫生—中药学]