苦参碱和氧化苦参碱对SMMC-7721细胞增殖及Stat3,Stat5基因表达的影响  

Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line

作　　者：郑艳敏[1] 李轩[1] 赵红艳[1] 赵军艳[1] 

机构地区：[1]武警医学院附属医院消化内科,天津300162

出　　处：《中国中药杂志》2008年第19期2234-2237,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察苦参碱和氧化苦参碱对肝癌细胞SMMC-7721增殖及Stat3,Stat5基因表达的影响。方法:苦参碱和氧化苦参碱干预肝癌细胞SMMC-7721,MTT法检测其对肝癌细胞增殖的影响,双荧光染色观察细胞凋亡率,RT-PCR法检测其对SMMC-7721细胞Stat3,Stat5 mRNA的影响。结果:苦参碱和氧化苦参碱作用于肝癌细胞后,能显著抑制肝癌细胞增殖,促进其凋亡,并呈时间剂量依赖性;二者均能下调Stat3,Stat5 mRNA表达水平。以相同浓度苦参碱和氧化苦参碱比较,苦参碱对肝癌细胞Stat3,Stat5 mRNA下调作用更显著(P<0.05或P<0.01)。结论:苦参碱和氧化苦参碱能显著抑制SMMC-7721细胞增殖,其机制可能与下调Stat3,Stat5的基因表达,抑制细胞信号传导通路有关。Objective： To study the effect of matrine and oxymatrine on proliferation and the expression of Stat3, Stats mRNA in SMMC-7721 cell line. Method： Treated with matrine and oxymatrine, the inhibitory effect on SMMC-7721 cell proliferation was detected by MTT, double fluorescence labeling was applied to measure the apotosis ratios of SMMC-7721 cells, the expression of Stat3 and Stats mRNA in SMMC-7721 cell line were assessed with RT-PCR. Result： Matrine and oxymatrine could inhibit the proliferation and induce the apoptosis of SMMC-7721 cells and it was time and dose dependent, the expression of Stat3 and Stats mRNA in SMMC- 7721 cell with matrine and oxymatrine were significantly lower than those in control group （P 〈 0. 05 or P 〈 0. 01 ）. Compared with the same dose of matrine and oxymatrine, matrine showed stronger effect on cell proliferation, apoptosis, and Stat3 and Star5 mRNA （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： Matrine and oxymatrine inhibited the proliferation and induced the of SMMC-7721 cells significantly, the mechanism of which might be related to the down-regulation of stat3 and star5 mRNA and inhibition of the signaling transduction pathway.

关 键 词：苦参碱 氧化苦参碱 信号传导 STAT3 STAT5 

分 类 号：R285.5[医药卫生—中药学]