人正常骨髓CD34^+造血细胞体外扩增形成粒单系造血祖细胞的能力  

Ex vivo expansion of human bone marrow CD34+ hematopoietic cells captured in strornal cells-free liquid culture system

作　　者：奚永志[1] 孔繁华[1] 金荔[1] 陈兴国[1] 张双喜 唐佩弦 魏文 

机构地区：[1]军事医学科学院附属医院免疫学研究室,北京100039

出　　处：《中国病理生理杂志》1997年第6期574-578,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：CD34+造血细胞以其独特的生物学功能正成为造血调控、造血于细胞移植和基因治疗最理想的靶细胞。本文探讨人正常骨髓CD34+造血细胞在体外扩增形成单个核细胞（MNC）及粒单系集落形成单位（CFU－GM）的能力，采用CIMS－100新型免疫磁性无菌分离术可获得纯度＞90％的CD34+造血细胞，其直接形成CFLJ－GM的能力要较骨髓MNC提高的倍，并且在含EGIIS组合造血生长因子的无基质液培养系统中，CD34+造血细胞在四周内可持续扩增产生大量MNC及CFU－GM，最高分别可达1770倍和48倍，但不同个体问CD34+造血细胞的这种能力差别较大。上述结果提示，CD34+造血细胞在最佳组合HGFs的无基质液培养条件下，能扩增产生大量成熟及晚期造血祖细胞，可以适用于临床治疗的需求，The expansive potentials of MNC and CFU - GM derived for CD 34+ hematopoietic cells of human bone were determined. With CIMS - 100 inmunomagnetic isolator, a novel sterilized separation systm, more than 90% purity of CD 34+ hematopoietic cells could be obtained. Freshly purified CD 34+ hematopoietic cells lead to 48 - fold increase in CFU- GM over that observed with BMMNC. Highly purified CD 34+ hematopoietic cells were then culture in a stromal cells - free liquid culture system (SCFLCS) supplemented with EPO + GM - CSF + IL 3 + IL 6 + SCF(EGllS). The cells harvested from SCFLCS were counted and assayed CFU - GM at weekly interval for a pened of 4 weeks. CD 34+ hematopoietic cells from all of 5 cases were able to produced continuously a significant amounts of MNC and CFU -- GM with the most dramat incest of 1770 - fold and 48-fold during a pened of 4 weeks. Our data suggested the feasibility of large - scale expansion of CD 34+ hematopoietic cells stalting from small numbers in SCFLCS supplermted with EGILS combinations.

关 键 词：造血干细胞 培养基 骨髓 体外扩增 靶细胞 

分 类 号：R457.7[医药卫生—治疗学] R329-33[医药卫生—临床医学]