聚合酶链反应检测ApoE基因型的研究  

A methodological study for apolipoprotein E genotype using the polymerase chain reaction

作　　者：周新[1] 涂建成[1] 哈黛文[1] 贺小玲[1] 吕民 

机构地区：[1]湖北医科大学第二临床学院医学检验系,武汉430071

出　　处：《中国病理生理杂志》1997年第6期740-743,共4页Chinese Journal of Pathophysiology

摘　　要：本文以人工合成的具有3’-末端错配的寡核苷酸引物，进行聚合酶链反应（polymerasechainreaction,PCR），检测104份健康成人血脱辅基脂蛋白E（apolipoproteinE，ApoE）基因型，共检出5种常见的ApoE基因型，以ApoE3／3型最多，占总数的月71.2％，等位基因频率分布以ε3（0．8465）最高。PCR法的模板DNA取自血白细胞，与常规的等电聚焦电泳（isoelectrofocusing,IEF）比较，36份健康成人血样品同时用两种方法作ApoE分型，结果基本一致，对比分析，PCR法检测ApoE基因型，具有快速灵敏及特异性高等优点。Phenotyping ApoE was studied by polymerase chain reaction(PGR). Synthetic allele(specific oligonucleotide primers)with base-pair mismatched at 3'-terminal to amplify. ApoE gene fragment were used in this method. 104 healthy normolipid adults were detected(tolal 5 types). It was found that ApoE3/3 was 71. 2%, and d, the hightest allele frequency distribution(0. 8465 ). The templete DNA used to amplify was isolated from human white blood cell. ApoE phenotypes were detected with both IEF (isoelectrofocusing) and PCR in 36 healthy adults. Data obtained with these two methods accorded with each other, but in comparison with IEF, the PCR methed was un sensitive, specific and rapid.

关 键 词：动脉粥样硬化 高脂血症 载脂蛋白 APOE PCR 

分 类 号：R543.5[医药卫生—心血管疾病] R446.9[医药卫生—内科学]