血液透析患者血清Fractalkine、TIMP-1水平的变化及意义  

The significance of serum fractalkine and TIMP-1 changes in maintenance hemodialysis patients

作　　者：王玲[1] 林洪丽[1] 赵光本[1] 乔世岩[1] 王可平[1] 杭宏东[1] 于长青[1] 

机构地区：[1]大连医科大学附属第一医院肾内科

出　　处：《中国血液净化》2008年第10期542-545,共4页Chinese Journal of Blood Purification

基　　金：国家自然科学基金资助项目(30470805)

摘　　要：目的测定维持性血液透析(MHD)患者血清Fractalkine(FKN)及金属蛋白酶组织抑制剂-1(TIMP-1)水平的变化,探讨FKN、TIMP-1与炎症的关系。方法选取长期规律血液透析(HD)的患者30例,应用ELISA法检测其透析前、后的FKN、TIMP-1血清水平,应用免疫散射比浊法测定透析前血清超敏C反应蛋白(Hs-CRP)水平,同时检测其血白蛋白(ALb)、血红蛋白(Hb)、体重指数(BMI)、透析充分性指标(Kt/V)及透析前后尿素氮(BUN)、肌酐(Scr)水平。结果MHD患者HD后的血清FKN水平比HD前升高(P<0.01),HD后的TIMP-1水平比HD前升高(P<0.01)。HD前血清FKN水平与HD前Hs-CRP呈正相关(r=0.387,P<0.05),HD前血清FKN与HD前TIMP-1、透析年限无相关性(P>0.05);HD前TIMP-1水平与HD前Hs-CRP、透析年限无相关性(P>0.05)。HD前后FKN差值与HD前Hs-CRP呈正相关(r=0.291,P<0.05),与HD前后TIMP-1差值及透析年限无相关性(P>0.05);透析前后TIMP-1差值与HD前Hs-CRP呈正相关(r=0.226,P<0.05),与透析年限无相关性(P>0.05)。结论血液透析患者透析后血清FKN及TIMP-1水平增高。HD前后FKN及TIMP-1水平的瞬时变化可能与炎症有关。Objective To measure the changes of serum fractalkine（FKN） and tissue inhibitors of metalloproteinase-1（TIMP-1） in maintenance hemodialysis（MHD） patients,and to discuss the relationship be-tween inflammation and serum concentrations of FKN and TIMP-1.Methods We measured serum FKN and TIMP-1 by ELISA before and after hemodialysis in 30 MHD patients.Their serum high-sensitivity C-reactive protein（Hs-CRP） before hemodialysis was measured by immunonephelometry.Serum albumin（Alb）,hemoglo-bin（Hb）,body mass index（BMI） and the dialysis adequacy marker Kt/V were also determined.BUN and serum creatinine（Scr） were assayed before and after hemodialysis.Results Serum FKN and TIMP-1 increased after hemodialysis（P〈0.01,as compared with their respective values before hemodialysis）.Before hemodialysis,their serum FKN and Hs-CRP were positively correlated（r =0.387,P〈0.05）,but their serum FKN did not correlated to serum TIMP-1 or to hemodialysis duration（P〉0.05）,nor did their serum TIMP-1 to Hs-CRP or to dialysis duration（P〉0.05）.The differences of serum FKN concentrations before and after hemodialysis were positively correlated to Hs-CRP（r= 0.291,P〈0.05）,but had no correlation with the differences of serum TIMP-1 concen-trations before and after hemodialysis or to dialysis duration（P〉0.05）.The differences of serum TIMP-1 concen-trations before and after hemodialysis were also positively correlated to Hs-CRP（r=0.226,P〈0.05）,and had no correlation with dialysis duration（P〉0.05）.Conclusion Serum FKN and TIMP-1 increase after hemodialysis in MHD patients.The instantaneous changes of serum FKN and TIMP-1 after hemodialysis may relate to the microinflammatory state.

关 键 词：金属蛋白酶组织抑制剂-1 血液透析 FRACTALKINE 微炎症状态 

分 类 号：R318.16[医药卫生—生物医学工程]