产氢光合细菌的分离鉴定及高产氢菌株的筛选  

Isolation and Identifi cation of Photosynthetic Bacteria and Their Hydrogen-producing Abilities

作　　者：王永忠[1] 廖强[1] 朱恂[1] 田鑫[1] 王中康[2] 张攀[1] 张宝鹏[2] 

机构地区：[1]重庆大学工程热物理研究所,重庆400030 [2]重庆大学生物工程学院,重庆400030

出　　处：《应用与环境生物学报》2008年第5期673-677,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(Nos.90510020;50576107);教育部新世纪优秀人才支持计划(No.NCET-04-0845)~~

摘　　要：利用特殊培养基从光照充裕、有机质含量高的污水沟、稻田淤泥中富集培养得到光合细菌优势菌种,采用梯度稀释法,通过双层固体平板进行分离和纯化,得到4株具有产氢性能的光合细菌菌株,按照《伯杰细菌鉴定手册》(第8版)关于光合细菌分类的方法进行生理生化特征鉴定,进行了16SrDNA的基因序列分析,并与NCBI上标准菌株基因序列进行比对,确定CQU-Z、GLS-Z、CQK-Z、CJK-C的16SrDNA碱基长度分别为1368bp、1453bp、1369bp、1432bp,结果表明,这4株光合细菌菌株分别属于Rhodopseudomonas palustris和R.gelatinosa.同时通过光生物反应器进行产氢性能研究,表明光照条件为590nm、6000lx时,菌种CQK-Z在连续62h培养中总产氢量最高,为12.04mmol,相应的光化学效率和最大产氢速率分别为33.45%、1.84mmolL-1h-1.Using special culture medium, dominant photosynthetic bacteria （PSB） were enriched from sewage silt and paddy fields with plentiful light and organic compounds. Four strains of photosynthetic bacteria capable of producing hydrogen were purified and isolated by gradual dilution and double-deck solid medium. The analysis of the gene sequence information of 16S rDNA was carried out, and it indicated that the strains of CQU-Z, GLS-Z, CQK-Z and CJK-C possessed 1 368 bp, 1 453 bp, 1 369 bp, 1 432 bp, respectively. According to taxonomy of the Bergey＇s Manual of Determinative Bacteriology （the 8^th edition） and comparison with corresponding standard strain＇s gene sequence in NCBI, the four strains were identified as Rhodopseudomonas palustris and R. gelatinosa, respectively. Moreover, it was found that when continuously cultured for 62 h with light of 590 nm and 6 000 Ix, the hydrogen yield by strain CQK-Z was maximum, 12.04 mmol, and its photo-chemical efficiency of light energy and the maximum production rate of hydrogen was 33.45% and 1.84 mmol L^-1 h^-1, respectively.

关 键 词：光生物制氢 分离 鉴定 RHODOPSEUDOMONAS palustris Rhodopseudomanas gelatinosa 

分 类 号：Q93[生物学—微生物学]