克拉维酸产生菌的诱变育种  

Mutagenesis Breeding of Clavulanic Acid-producing Streptomyces clavuligerus

作　　者：孙荣勋[1] 韩斌 赵海[1] 范成英[1] 官家发[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]东莞市保得生物工程有限公司,广东东莞523087

出　　处：《应用与环境生物学报》2008年第5期684-687,共4页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-YW-G-008)~~

摘　　要：以克拉维酸产生菌棒状链霉菌Streptomyces clavuligerus CCRC11518 III50为出发菌株,比较各种物理和化学诱变剂处理对其克拉维酸生物合成的影响,确定亚硝基胍为棒状链霉菌诱变育种的诱变剂及其处理剂量为2mgmL-1、40min.经亚硝基胍处理40min(处理浓度为2mgmL-1)后,采用新颖理性化筛选方法,通过筛选自身代谢产物抗性突变株、克拉维酸抗性突变株和链霉素抗性突变株,最终得到一株克拉维酸高产菌VI118,其克拉维酸产量较出发菌株提高了67.9%.该高产突变株在琼脂斜面培养基上连续传接10代,克拉维酸产量保持稳定.Using a novel rational screening method, Streptomyces clavuligerus CCRC11518 III50, as a clavulanic acid-producing parent strain, was treated by NTG （2 mg mL^-1） for 40 min, and the self-generated metabolite resistant mark, clavulanic acid resistant mark and streptomycin resistant mark were added step by step. Finally, mutant VI.8 with the three marks was obtained. The yield of clavulanic acid by this mutant was increased by 167.9% compared with that by its parent strain. After 10 generations on the agar medium slant, the production of clavulanic acid by this mutant was still stable.

关 键 词：棒状链霉菌 克拉维酸 理性化筛选 菌种改良 

分 类 号：Q93[生物学—微生物学]