2型糖尿病患者外周血淋巴细胞G蛋白偶联受体激酶2基因表达升高  

An increased expression of G protein-coupled receptor kinase 2 gene in circulating lymphocytes in type 2 diabetes patients

作　　者：陈红梅[1] 林秋雄[1] 谭虹虹[1] 杨华章[2] 余细勇[1] 

机构地区：[1]广东省人民医院医学研究中心,广州510080 [2]广东省人民医院内分泌科,广州510080

出　　处：《中国糖尿病杂志》2008年第10期604-606,共3页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(30571850;30300421);广东省自然科学基金团队资助项目(20015015)

摘　　要：目的探讨糖尿病对G蛋白偶联受体激酶2(GRK-2)基因的影响及其机制。方法56例40~65岁非高血压2型糖尿病(T2DM)患者为T2DM组,以年龄、性别相匹配的高血压糖尿病患者46例(HT-DM组)和体检健康者29例(NC组)为对照组,提取外周血淋巴细胞RNA,通过RT-PCR半定量检测GRK-2 mRNA表达。结果与HT-DM组相似,T2DM组外周血淋巴细胞GRK-2 mRNA表达水平较NC组明显增加(P<0.05)。Pearson线性相关分析提示T2DM组患者GRK-2基因表达水平升高与PG、HbA1c、糖尿病病程、年龄、TC水平正相关(P<0.05)。结论T2DM患者外周血淋巴细胞GRK-2基因表达升高,血糖升高可能是其重要原因。Objective Cardiac beta-adrenoceptor signaling was impaired in heart of diabetes patient and rat. The process of beta-adrenoceptor desensitization is initiated by a family of serine/threonine kinases （GRKs）. G-protein coupled receptor kinase-2 （GRK-2） has been proved to have over-expression in circulating lymphocytes of heart disease patient. Thins study was to observe GRK-2 mRNA expression in diabetes patient. Methods 56 patmnts with type 2 diabetes who were aged 40-65 years, without hypertension and heart diseases were observed. 46 patients with type 2 diabetes who complicated hypertension and 29 health subjects were used as controls. Lymphocyte GRK-2 mRNA level was detected by RT-PCR. Results Similar to type 2 diabetes panents with hypertension,the GRK-2 mRNA level in non-hypertensive type 2 diabetes panents was higher than that in the health controls（P^0.05）. The GRK- 2 mRNA level was correlated with age,course, glucose,glycosylated hemoglobin and total cholesterol in non-hypertensive type 2 diabetes subjects （P〈0.05）. Conclusions Our data show GRK-2 expression increases＇in lymphocytes of type 2 diabetes patients without hypertension, and the excessive GRK-2 gene expression may be the cause of heart damage in diabetes.

关 键 词：糖尿病 G蛋白偶联受体激酶2 临床分析 治疗方法 

分 类 号：R587.1[医药卫生—内分泌] R593.241[医药卫生—内科学]