绞股蓝多糖对HepG_2细胞酒精性损伤的保护作用  

Protective effect of Gynostemma Pentaphyllum polysaccharide on alcohol-induced injury of HepG_2 cell

作　　者：宋淑亮[1] 肖增平[1] 吉爱国[1] 梁浩[1] 王伟莉[1] 王允山[1] 

机构地区：[1]山大威海国际生物技术研发中心,山东威海264209

出　　处：《中国生化药物杂志》2008年第5期302-305,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：威海市大学共建项目(编号:0000413420615)

摘　　要：目的研究绞股蓝多糖对HepG2细胞酒精性损伤的保护作用及可能作用机制。方法在HepG2细胞培养液中加入不同浓度的绞股蓝多糖,作用5h后,再加入无水乙醇,建立细胞损伤模型,继续培养12h,四甲基偶氮唑盐(MTT)法检测细胞活性,同时比较各组细胞丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、丙二醛(MDA)和超氧化物歧化酶(SOD)活性的变化;Hoechst33342进行细胞凋亡形态学染色。结果不同浓度的绞股蓝多糖均可以提高损伤后HepG2细胞的存活率,抑制AST、ALT水平的升高,减少MDA的形成,提高SOD的活性,减少细胞凋亡。结论低剂量的酒精能引起HepG2细胞的凋亡,绞股蓝多糖对HepG2细胞酒精性损伤具有保护作用,其机制可能与清除氧自由基、对抗脂质过氧化有关。Purpose To study the protective effect and mechanism of C, ynostemma Pentaphyllum polysaceharide on alcohol-induced injury of HepG2 cell. Methods Different concentrations of Gynosterrtma Pentaphyllure polysaecharide were added into the culture medium, which was then treated with alcohol to form the injury model. The survival rate of HepG2 cell was determined at 12 h by MTT method. The levels of ALT, AST, MDA and SOD after cultivation for 12 h were determined respectively. Apoptotic cells were identified by Hoeehst33342 staining. Results All doses of Gynostemma Pentaphyllum polysacchafide could significantly improve the survival rate of injured HepG2 cell, inhibit the enhanced activities of ALT and AST, and inhibit MDA production and the decreasing of SOD induced by alcohol injury. Conclusion Low dose of alcohol can induce the apoptosis of HepG2 cell obviously. Oynostemma Pentaphyllum polysacchafide could effectively protect HepG2 cell from alcohol-induced injury, which is possibly associated to its clearance of oxygen free radicals and its anti-lipid peroxidation.

关 键 词：绞股蓝多糖 HEPG2细胞 肝损伤 保护作用 

分 类 号：R965[医药卫生—药理学]