核糖核酸Ⅱ制备及其病毒灭活工艺的验证  

Preparation of RNAⅡ and validation of its virus inactivation

作　　者：朱文赫[1] 孙妙囡[2] 孙德军[1] 

机构地区：[1]吉林大学再生医学科学研究所生物技术药物教研室 [2]吉林大学白求恩医学院,吉林长春130021

出　　处：《中国生化药物杂志》2008年第5期332-334,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的从牛胰脏制备核糖核酸Ⅱ(RNAⅡ),对巴氏灭活法灭活病毒工艺进行验证。方法以苯酚、氯仿抽提法制备,以猪细小病毒(PPV)为指示病毒,观察用巴氏灭活法(60℃,10h)灭活RNAⅡ原液中病毒的效果,用纯度、A260/A280比值、紫外特征峰、完整性、含量变化考察灭活前后制剂变化。结果制备了纯度和活性均较好的RNAⅡ,PPV滴度为7.7logTCID50/0.1mL时灭活液中未检测出PPV,经盲传3代,亦未发现特异性细胞病变,灭活前后制剂无质的变化。结论可以现行方法从牛胰脏制备较高纯度RNAⅡ,巴氏法能对RNAⅡ溶液作有效的病毒灭活处理。Purpose To purify RNA Ⅱ from calf pancreas and to validate the virus inactivationl by pasteurization. Methods RNA Ⅱ was purified from pancreas by extraction of phenol and chloroform and RNA solution adding the porcine parvovims （PPV）was heated to 60℃ for 10 hours. Purity, A260/A280, special UV absorbanee peak and integrity were matched before and after pasteurization. Results High purity and activity of RNA Ⅱ were purified, and the initial titre of PPV added into RNA Ⅱ solution is 7.71ogTCID50/0.1 mL. The residual virus was undeteetable in the virus inactivation solution and the matched itoms didn＇t change qualitatively. Conclusion The method of purification of RNA Ⅱ and inactivation of virus is effective.

关 键 词：核糖核酸Ⅱ 制备 病毒灭活 巴氏法 

分 类 号：TQ464.5[化学工程—制药化工]