皮肤烧伤愈合过程中磷酸化JNK的变化  

Changes of Phospho-JNK Expression during the Skin Burned Wound Healing

作　　者：熊昌艳[1] 官大威[1] 刘志会[2] 甄博[1] 赵锐[1] 朱宝利[1] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]武警辽宁总队医院烧伤科,辽宁沈阳110034

出　　处：《法医学杂志》2008年第5期325-326,335,401,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30271347);教育部留学归国基金资助项目(教外留司[2000]367)

摘　　要：目的观察烧伤后人皮肤磷酸化JNK(p-JNK)的变化特点,初步探讨烧伤后创面愈合的分子机制。方法取12例烧伤后住院植皮患者的烧伤周边区皮肤为烧伤周边皮肤组;另取正常皮肤12例为正常皮肤组。应用免疫组织化学方法和常规HE染色检测p-JNK在皮肤烧伤周边区和正常皮肤组织中的变化情况。结果正常皮肤组织中表皮基底层细胞的胞浆和胞核内有p-JNK阳性着色,阳性细胞率为(8.8±1.3)%。在烧伤周边皮肤组中,p-JNK阳性着色主要定位于表皮细胞和部分炎症细胞中,阳性细胞率上升至(31.2±3.3)%,明显高于正常皮肤组织(P<0.01)。结论烧伤后人皮肤p-JNK的变化可能与创面愈合有关。（p-JNK） Objective during the burned wound healing To observe the changes of expression of phosphorylation c-Jun NH2-terminal kinase skin burned wound healing in patients and discuss the molecular mechanism of Methods The staining intensity and distribution of p-JNK were detected by immunohistochemistry and routine histology in burned skin samples of 12 patients and normal skin samples of 12 control subjects. Results In normal skin, the positive signals of p-JNK were mostly localized in basal layer cells of the epidermis, with a positive rate of （8.8_＋1.3）%. In the burned group, the positive signals of p-JNK were mainly localized in the epidermal cells and some inflammatory cells, with a significantly higher positive rate of （31.2±3.3）% than the normal group（P〈0.01）. Conclusion The changes of p-JNK expression after skin burned might correlate with wound healing.

关 键 词：法医病理学 烧伤 JNK丝裂原活化蛋白激酶类 伤口愈合 

分 类 号：R644[医药卫生—外科学] R749.16[医药卫生—临床医学]