施氏鲟不同组织抗氧化酶对水体盐度升高的响应  

Responses of antioxidases in different tissues of Acipenser schrenckii to increased salinity in water

作　　者：赵峰[1] 庄平[1,2] 章龙珍[1] 黄晓荣[1] 张涛[1] 冯广朋[1] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海海洋大学上海高校水产养殖学E-研究院,200090

出　　处：《海洋水产研究》2008年第5期65-69,共5页Marine Fisheries Research

基　　金：国家863高技术研究发展计划项目(2004AA603110、2008AA10Z227);国家自然科学基金项目(30490234);上海市教委E-研究院项目(E03009);农业部海洋与河口渔业重点开放实验室项目(开-1-04-08)资助

摘　　要：研究测定了盐度驯化过程中施氏鲟不同组织中超氧化物歧化酶(Superoxide dismutase,SOD)和过氧化氢酶(Catalase,CAT)的活力变化,SOD和CAT以肝脏中含量最高,随着盐度升高,SOD活力呈下降趋势,显著低于对照组(P<0.05)。随着驯养时间的延长,除心脏和肾脏外,其他组织器官中SOD活力均有所回升。不同盐度下,心脏中CAT活力无显著差异(P>0.05),均显著低于对照组(P<0.05);肝脏、脾脏和肾脏组织中CAT活力变化趋势基本相同,呈先下降后上升的趋势。其中盐度25下,肝脏和肾脏CAT活力显著高于对照组(P<0.05)。本研究表明,盐度对施氏鲟不同组织中SOD和CAT活力具有抑制作用,但随着盐度驯养时间的延长,SOD及CAT的活力会有不同程度的恢复。Activities of SOD and CAT in different tissues of Acipenser schrenckii at salinities of 10, 20 and 25 during salinity acclimation were measured. In this study, SOD and CAT activities in liver tissue were the highest among the tissues tested. SOD activity in different tissues declined with the increasing salinity, which is significantly different from that in the control. With the increase of salinity acclimation time, activity of SOD in different tissues gradually increased, except in heart and kidney. CAT activity in different tissues tested at salinity 10 was significantly （P〈0.05） lower than that in the control. At salinities of 10, 20 and 25, there were no significant difference （P〉0.05） in CAT activity in heart tissue. But they were all lower than that in the control group. Activity of CAT in liver, spleen and kidney showed the same pattern of change, decreased first and then increased. At salinity of 25, CAT activities in liver and kidney tissues were significantly higher （P〈0.05） than those in the control group. The study showed that SOD and CAT in different tissues of A. schrenckii were inhibited first by the increasing salinity and then recovered, to some extent, with the elapse of salinity acclimation time, which maybe attributed to the osmoregulation ability of A. schrenckii.

关 键 词：施氏鲟 超氧化物歧化酶 过氧化氢酶 盐度驯化 

分 类 号：Q959.463[生物学—动物学] S917[农业科学—水产科学]