HPLC法检测细辛脑注射液的有关物质  

HPLC determination of the related substances of asarone injection

作　　者：李少刚[1] 郭欢[2] 郭小青[3] 

机构地区：[1]山西医科大学第二医院,山西太原030001 [2]太原市万柏林区中心医院,山西太原030024 [3]山西大学中医药现代研究中心,山西太原030006

出　　处：《临床医药实践》2008年第10期850-851,共2页Proceeding of Clinical Medicine

摘　　要：目的:建立细辛脑注射液有关物质的高效液相色谱(HPLC)检测方法。方法:采用Diamonsil-C18色谱柱(200 mm×4.6 mm,5μm);流动相A为甲醇,流动相B为十二烷基硫酸钠、磷酸二氢钾水溶液,采用梯度洗脱,10 min后流动相A在35 min内从60%升至80%,维持5 min,流速1.0 mL/min,检测波长258 nm。结果:细辛脑的最低检测限量25 ng,细辛脑与各杂质分离良好。结论:本法简便快速,灵敏度高,准确可靠。Objective：To establish a HPLC method for detection of the asarone injection＇s related substances. Methods ：The analytical column was Diamonsil-C18 （200 mm × 4. 6 mm, 5 μm）. Using gradient elution method, the mobile phase A was methanol and the mobile phase B was lauryl sodium sulfate ,potassium dihydrogen phosphate water-soluble fluid （ten minutes later,the mobile phase A rose from 60% to 80% in thirty-five minutes,kept five minutes ）. The flow rate was 1. 0 mL/min. The detection wavelength was 258 nm. Results：The detection limits for asarone were 25 ng respectively. Good resolution for asarone and related substances was obtained. Conclusion;The method is simple ,rapid,sensitive and accurate.

关 键 词：细辛脑注射液 有关物质 高效液相色谱法 梯度洗脱 

分 类 号：R817[医药卫生—影像医学与核医学]