精子核成熟度与精子形态的关系  

Research on the relationship between sperm nuclear mature and sperm morphology

作　　者：王洪亮[1] 王瑞雪[2] 许宗革[2] 刘睿智[2] 

机构地区：[1]吉林大学第一医院泌尿外科,吉林长春130021 [2]吉林大学基础医学院细胞生物学教研室

出　　处：《中国妇幼保健》2008年第30期4305-4307,共3页Maternal and Child Health Care of China

基　　金：吉林省科技厅资助项目(项目编号:200505140)

摘　　要：目的:研究精子核成熟度与精子形态关系,探讨精子形态异常机制。方法:收集55例精液样本,其中生育组19例,畸形精子症组36例。应用CASA分析精子密度、活力,伊红染色检测精子活率,联苯胺染色分析精液白细胞浓度,改良巴氏染色分析精子形态,苯胺蓝染色法评价精子核成熟度。结果:畸形精子症组苯胺蓝染色阳性率显著高于生育组(P<0.05)。头部异常、颈部异常、尾部异常、无定型、其它畸形精子组苯胺蓝染色阳性率显著高于形态正常精子组苯胺蓝染色阳性率(P<0.05)。结论:精子核成熟异常可导致精子形态异常。Objective： To investigate the relationship between sperm nuclear maturity and sperm morphology and explore the mechanism of the abnormal morphological sperm. Methods： 55 Semen samples were collected, including 36 teratozoospermia men and 19 fertile men. Sperm concentration and motility were analyzed with CASA. Sperm viability was detected after Eosin staining. Semen WBC concentration was tested after Benzedrine staining. Sperm morphology were analyzed according to Modified Papanicolaou staining. Sperm nuclear matu- rity was evaluated using aniline blue staining, Results： The percentage of sperm with positive aniline blue stain in tcratozoospermia group was significantly higher than that in control group （ P 〈 O. 05 ） . Comparing with normal morphological sperm group, the rate of sperm with positive aniline blue stain significantly increased in head defects sperm group, as well as in neck/midpiece defects sperm group, tail defects sperm group, irregularity sperm group and other abnormal sperm group （ P 〈 0. 05 ） . Conclusion： Sperm nuclear maturity will be related with sperm morphology and abnormal sperm nuclear maturity can result abnormal morphological sperm.

关 键 词：精子核 成熟度 精子形态 

分 类 号：R697[医药卫生—泌尿科学]