经前期综合征模型大鼠下丘脑和外周血内吗啡肽含量变化的实验研究  

Study on the content of endomorphin in the hypothalamus and serum of premenstrual syndrome model rats

作　　者：马晶[1] 张惠云[1] 

机构地区：[1]山东中医药大学科技处,济南250355

出　　处：《药学与临床研究》2008年第5期343-346,共4页Pharmaceutical and Clinical Research

基　　金：国家自然科学基金资助项目(批准号:30572291)

摘　　要：目的:检测经前期综合征(PMS)肝气逆、肝气郁证模型大鼠下丘脑及外周血内啡肽(EM)含量变化,观察经前平、经前舒颗粒对PMS肝气逆、肝气郁证的干预作用。方法:以情志刺激多因素法复制PMS肝气逆、郁证大鼠模型,以经前平、经前舒颗粒干预治疗,采用放射免疫法检测模型大鼠下丘脑及血清EM-1、EM-2的含量。结果:与正常组相比,PMS肝气逆证组下丘脑EM-1含量无显著性变化,血清EM-1含量显著上升(P<0.01);PMS肝气郁证组下丘脑EM-1含量亦无显著性变化,血清EM-1、EM-2含量显著下降;与模型组相比,经前平组血清EM-1、EM-2含量显著性下降(P<0.01),经前舒组血清EM-1、EM-2显著上升。结论:两种病证模型下丘脑EM-1、EM-2的含量变化不明显;PMS肝气逆证大鼠血清EM-1显著性升高,EM-2有上升趋势;PMS肝气郁证大鼠血清EM-1、EM-2含量显著性下降。经前平、经前舒颗粒可分别对外周血中指标的异常变化起到调节作用。Objective： To detect the content change of endomorphin （EM） in the hypothalamus and serum of the liver-qi invasion and the liver-qi depression of premenstrual syndrome PMS model rats, and observe the intervention effect of the Jingqianping and Jingqianshu granule. Methods： PM5 model rats with liver-qi invasion were prepared by electric stimulating. PMS model rats with liver-qi depression were prepared by binding the limbs. The content of EM was examined by the radio immunoassay. Results： Compared with the normal group, the content of EM-1 has no significant changes in the hypothalamus, but increased markedly in the serum（P 〈0. 01 ）of PMS model rats with liver-qi invasion, and the content of EM- 1 and EM-2 also have no significant changes in the hypothalamus, hut decreased markedly （P 〈0.01, P 〈 0.05） in the serum of liver-qi depression model rats; the content of EM-1 decreased markedly（P 〈0.0t ） in the serum of Jingqianping granule group; the content of EM-1 and EM-2 increased markedly（P 〈0.01, P 〈 0. 05）in the serum of Jingqianshu granule group. Conclusion： The content of EM-1 ,EM-2 have no significant changes in the hypothalamus of PMS model rats, but increased markedly in the serum of PMS model rats with liver-qi invasion, while decreased significantly in the serum of liver-qi depression model rats, and the two medicine may cure the diseases by regulating the above abnormal changes.

关 键 词：经前期综合征 PMS肝气逆证/肝气郁证 内啡呔 

分 类 号：R965.1[医药卫生—药理学]