康尔心胶囊中三七和人参的有效成分含量测定  

Determination of the active compounds of Panax notoginseng and Panax ginseng in Kangerxin capsules

作　　者：赵连中 姚丹丹 葛玉松 罗海燕[2] 

机构地区：[1]江苏省盐城药品检验所,盐城224002 [2]南京中医药大学中药学院,南京210029

出　　处：《药学与临床研究》2008年第5期370-372,共3页Pharmaceutical and Clinical Research

摘　　要：目的：建立康尔心胶囊中三七和人参的有效成分含量测定方法。方法：Diamonsil C18柱（250 mm×4.6 mm,5μm）,以乙腈为流动相A,水为流动相B,梯度洗脱。线性梯度为：0～9.5 min,A27%;9.5～15 min,A27%～45%;15～22 min,A45%;22～23 min,A45%～27%;23～30 min,A27%。流速：1.0 mL.min^-1;柱温：35℃。结果：人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1线性范围分别依次为0.039 6～0.158 4μg、0.040 76～0.163 04μg、0.041 2～0.164 8μg;平均回收率分别为100.2%、100.1%、99.7%,RSD分别为0.38%、0.39%、0.36%。结论：本方法简便可靠,结果稳定,可用于康尔心胶囊中三七和人参的有效成分含量测定。Objective： To develop a method for the determination of active compounds of Panax notoginseng and Panax ginseng in Kangerxin capsules. Methods： The diamonsil C18 column （250 mm ×4.6 mm, 5 μm） was used with acetonitrile as the mobile phase A and water as the mobile phase B. The linear grade is 0 9. 5min, A 27% ; 9. 5 ～ 15 min, A 27% - 45% ; 15 - 22 min, A 45% ; 22 - 23min, A 45% - 27% ; 23 30 min, A27%o The flow rate was 1.0 mL·min^-1. The temperature of column is 35℃. Results： The linear ranges of ginsenoside Rgl, Rbl, notoginsenoside R1 in Kangerxin capsules were 0. 039 6 -0. 158 4μg, 0.040 76 -0.163 04μg, 0.041 2 -0. 1648 μg respectively. The average recoveris were 103.20%, 100. 13%, 99.68% respectively. RSD were 0. 38% ,0. 39% ,0. 36% respectively. Conclusion： This method is convenient, reliable and stable for the determination of the quality control of Kangerxin capsules.

关 键 词：康尔心胶囊 人参皂苷RG1 人参皂苷RB1 三七皂苷R1 含量测定 

分 类 号：R927.2[医药卫生—药学]