孕鼠尾静脉注射脑源性神经营养因子对宫内发育迟缓新生大鼠体质量及脑质量的影响  

Effect of Brain Derived Neurotrophic Factor on the Birth Weight and the Brain Weight of Newborn Rats with Intrauterine Growth Retardation

作　　者：韩树萍[1] 王玢[1] 李亚琴[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院儿科,南京210004

出　　处：《实用儿科临床杂志》2008年第20期1590-1591,共2页Journal of Applied Clinical Pediatrics

基　　金：南京市医学科技发展基金重点项目资助(Zkx06011)

摘　　要：目的探讨孕鼠尾静脉注射脑源性神经营养因子(BDNF)对宫内发育迟缓(IUGR)新生大鼠体质量及脑质量的影响。方法选取适龄SD大鼠进行交配,以观察到阴道栓确定为妊娠第1天,随机分为IUGR组和假手术组,于孕第17天开腹行孕鼠双侧子宫动脉钳夹术,以微小动脉血管夹钳夹双侧子宫血管,30 min后恢复血供,手术缝合;假手术组孕鼠仅开腹不予血管钳夹。IUGR组术后孕鼠尾静脉注射BDNF 1μg(BDNF干预组)或9 g/L盐水(IUGR组),持续5 d。待自然分娩后对各组新生大鼠进行体质量及脑质量分析。结果与假手术组相比,IUGR组新生大鼠出生体质量减轻24.9%,脑质量减轻17.3%,差异均具有显著性(Pa<0.000 1);与IUGR组相比,BDNF干预组新生大鼠体质量增加约11.9%,脑质量增加约10.7%,差异均具有显著性(Pa<0.05)。结论孕鼠尾静脉注射BDNF干预可明显改善因IUGR所引起的新生大鼠体质量及脑质量减轻,提示外周静脉注射外源性BDNF,早期干预IUGR有一定效果。Objective To explore the effect of brain derived neurotrophic factor（BDNF） on the birth weight and the brain weight of newborn rats with intrauterine growth retardation（IUGR）. Methods Female and male Sprague - Dawley rats were mated together and the vaginal plug formation after mating was for pregnancy diagnosis. The day when the plug was observed was considered day 1 of gestation. Then, the pregnant rats were divided into IUGR group and sham surgery group. On day 17 of gestation, the uterine arteries of pregnant rats in IUGR group were clamped for 30 min by blood vessel forceps after abdominal cavity with clamping blood uessels was opened. The pegnant rats in sham surgery group only had abdominal cavity opened without clamping blood vessels. Then the pegnant rats in IUGR group received 9 g/L saline（ IUGR group） or BDNF 1 ug injected in the rat through caudal veins for 5 days after surgery（ BDNF intervention group）. After vaginal birth, the weight of newborn rats and their brain weigh were measured and analyzed. Results Compared with the sham surgery group, the birth weight and brain weight of IUGR group group rats decreased by 24.9% and 17.3%, respectively, the results were significant（ Pa 〈 0. 000 1 ）. And the weight of newborn rats and their brain of BDNF intervention group was significantly higher than those in IUGR group （Pa 〈 0. 05 ）. Compared with the IUGR group,the birth weight and the brain weight of BDNF IUGR group rats increased 11.9% and 10.7% for IUGR. Conclusions BDNF injection by rat caudal veins can increase the birth weight and the brain weight of newborn rats suffered from intrauterine growth retardation,which provides the possibility of using BDNF for drug therapy intervention of IUGR in the future.

关 键 词：神经营养因子 宫内发育迟缓 大鼠 新生 体质量 脑 

分 类 号：R714.7[医药卫生—妇产科学]