合欢植株再生体系快速建立技术的初步研究  

Study on High Efficient Establishment of Regeneration System of Albizia julibrissin Duraxx

作　　者：夏时云[1] 周祥明[1] 付任胜[1] 

机构地区：[1]天津市农业生物技术研究中心,天津300384

出　　处：《天津农业科学》2008年第5期1-3,共3页Tianjin Agricultural Sciences

基　　金：天津市农业生物技术研究中心主任基金(2008-03A)

摘　　要：以合欢无菌播种苗下胚轴为外植体,对建立合欢高效植株再生体系进行了研究。结果表明,细胞分裂素(6-BA)和生长素(2,4-D)不同浓度组合影响合欢无菌播种苗下胚轴愈伤组织和不定芽的分化,其中较低浓度的6-BA和2,4-D配合可以显著提高不定芽的分化频率。合欢无菌苗下胚轴最佳愈伤组织和不定芽诱导培养基分别为:MS+6-BA 0.5 mg/L+2,4-D 0.3 mg/L,不定芽分化频率可达90.5%以上;最佳生根培养基为:1/2MS+IBA 0.3 mg/L,生根诱导率90%以上,移栽易获得成功。合欢无菌苗下胚轴高效植株再生体系的建立,为合欢的细胞工程和基因工程操作及新品种的快速繁育奠定了基础。Hypocotyl explants from A lbizia julibrissin Duraxx were cultured on MS medium supplemented with different combinations of plant growth regulators. The effects of different combinations of hormone on hypocotyls were observed. The results showed that different combinations of 6-BA and 2,4-D affected the differentiation of callus tissue and adventitious bud, and low concentration combinations could increase the differentiation frequency of bud differentiation. The optimal culture mediums for callus tissue and adventitious bud induction was MS＋6-BA 0.5 mg/L＋2,4-D 0.3 mg/L, differentiation frequency of bud differentiation was up to 90.5%; the optimal rooting medium was 1/2MS＋ IBA 0.3 mg/L, rooting rate was more than 90%, it was easy to successful transplanting. The high efficient establishment of regeneration system of Albizia julibrissin Duraxx laid the foundation for operation of cell and gone engineering and fast breeding of new variety in Albiziajulibrissin Duraxx.

关 键 词：合欢 体外培养 种植 培养基 植株再生 

分 类 号：Q943.1[生物学—植物学]