生物絮凝剂产生菌的N^＋注入诱变选育及其培养条件研究  

Research on breeding of bioflocculant-producing strains by N^+ implantation and their culture conditions

作　　者：李培睿[1,2] 张东辉[1] 李宗伟[1] 李宗义[1] 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052 [2]河南师范大学生命科学学院,新乡453002

出　　处：《高技术通讯》2008年第10期1071-1074,共4页Chinese High Technology Letters

基　　金：973计划(2004CB719604)资助项目

摘　　要：为获得高絮凝活性菌株,采用低能氮离子诱变方法,对生物絮凝剂产生菌FJ-7进行诱变选育,筛选得到一株絮凝活性高、遗传稳定性良好的突变株 NIM-192。发酵产絮凝剂曲线表明,其菌体生长速度稍慢于原始菌株,但絮凝活性一直高于原始菌株,絮凝率比原始菌株提高了34.26%。进一步研究表明,蔗糖、葡萄糖、半乳糖是NIM.192产生絮凝剂的适宜碳源,酵母膏:尿素为1:1的混合氮源为最佳氮源,培养基起始 pH 为7～9时,发酵液的絮凝效果最好。NIM-192, a bioflocculant-produeing strain with good genetic stability and high flocculating activity, was screened out through implanting N^＋ into FJ-7, its original strain. Its curve of bioflocculant-producing by fermentation showed the growing speed of NIM-192 was slower, but the flocculating activity of bioflocculant was higher than the original strain, and the flocculating rate of NIM-192 increased by 34.26 %. Further studies showed that sucrose, glucose, galactose were the suitable carbon sources, the optimum nitrogen source was the mixture of yeast extract and urea with the proportion of 1 ： 1, and the best flocculation effect was obtained when the initial solution pH was adjusted to 7 - 9.

关 键 词：N^+注入 生物絮凝剂 生物絮凝剂产生菌 诱变选育 絮凝活性 培养条件 

分 类 号：Q93[生物学—微生物学]