寰枢椎周围软组织损伤对小鼠记忆能力的影响  

Effect of Soft Tissues Injury around Atlantoaxial Joint on Memory Abilities of Mice

作　　者：朱彤彤[1] 韩向北[2] 郭亚雄[2] 李德龙[2] 赵丽娟[2] 

机构地区：[1]吉林大学白求恩医学院 [2]吉林大学白求恩医学院病理生理教研室

出　　处：《中国康复理论与实践》2008年第10期930-931,F0003,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：吉林省教育厅资助项目(No.2005201)

摘　　要：目的观察寰枢椎周围软组织损伤对小鼠学习记忆能力的影响。方法44只小鼠随机分为模型组和对照组,各22只。模型组小鼠利用手术方法破坏寰枢椎周围软组织建立颈椎稳定性异常病理模型;采用水迷宫试验、跳台试验和洞板试验测试其学习、记忆能力;HE染色观察脑海马细胞数量;免疫组织化学染色观察脑海马caspase-3的表达。结果与对照组相比,模型组小鼠水迷宫试验中游全程时间和错误次数明显增加(P<0.01);跳台试验中模型组小鼠反应期明显延长,潜伏期明显缩短,错误次数明显增加(P<0.01);洞板试验中模型组小鼠潜伏期延长,探洞次数明显减少(P<0.01)。HE染色模型组小鼠脑海马可见明显散在的或连续的神经细胞变性、死亡;免疫组织化学染色显示模型组小鼠脑海马caspase-3蛋白呈阳性表达。结论寰枢椎周围软组织损伤可致颈椎稳定性异常而促进小鼠脑细胞凋亡,导致学习、记忆功能明显减退。Objective To observe the effect of the soft tissues injury around atlantoaxial joint on memory abilities of mice. Methods 44 mice were divided into the model group and control group with 22 animals in each group. The soft tissues around atlantoaxial joint were injured in the model group by surgery between the first and the second cervical vertebrae. The changes of learning and memory of mice were observed with step down avoidance test, water maze test and hole board test; the expression of caspase-3 in hippocam pus was observed by immunohistochemical staining. Results Compared with the control group, escape latency was longer and wrong times were more in water maze test of the model group （P〈0.01）. In step down avoidance test of the model group, the reaction period prolonged and wrong times increased obviously （P〈0.01）. In hole board test, the latency period prolonged and the times of extending the holes decreased significantly （P〈0.01）. Degenerated or dead neuron in hippocampus of surgery treated mice was found scatteredly or continuously with HE staining, the expression of caspase-3 protein was showed positive with immunohistochemical staining. Conclusion The soft tissues injury arround atlantoaxial joint can induce abnormality of the stability of cervical vertebra, which aggravated the aging process of the mice and decreased abilities of learning and memory.

关 键 词：软组织 学习记忆 寰枢椎关节 

分 类 号：R684[医药卫生—骨科学] R285.5[医药卫生—外科学]