紫外分光光度法测定川明参中总香豆素类成分含量  

Determination of the Content of Total Coumarin in Chuanminshen Violaceum by UV Spectrophotometry

作　　者：谌立巍[1] 刘静[1] 赵波[1] 李宏[1] 张梅[1] 

机构地区：[1]成都中医药大学药学院,成都610075

出　　处：《成都医学院学报》2008年第3期188-190,共3页Journal of Chengdu Medical College

基　　金：四川省中医药管理局科研基金资助项目(基金编号2004A16)

摘　　要：目的建立川明参药材中总香豆素含量测定方法。方法采用紫外分光光度法,以8-羟基-5-甲氧基补骨脂素为对照品,检测波长为273 nm。结果在1.53～7.65μg/ml质量浓度范围内,对照品8-羟基-5-甲氧基补骨脂素的吸光度和浓度线性关系良好,r=0.9991。平均回收率为101.8%,RSD=2.37%(n=6)。结论该法简便,可靠,为有效控制川明参药材质量奠定了一定的基础。Objective To establish a method for determination of the content of total coumarin in Chuanminshen viloaceum. Methods With 8-hydroxy-5-methoxypsoralen as the standard, the Coumarin in C. viloaeeum was detected at 273 nm by UV spectropho tometry. Results The absorbency and concentration of 8-hydroxy-5-mcthoxypsoralen had a good linear relation in the range of 1.53 - 7.65 （ g/ml （ r = 0.9991 ）. The average recovery rate was 101.8 %, RSD = 2.37 % （ n = 6 ）. Conclusion This method of content determination is simple and repeatable. Our research has laid a foundation for the quality evaluation on crude chuanminshen.

关 键 词：川明参 总香豆素 紫外分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学]