一种测定三磷酸腺苷二钠的新荧光光度法  

Determination of Adenosine Disodium Triphosphate by a Novel Fluorescence Spectrometric Method

作　　者：苗延虹[1] 侯法菊[2] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271000 [2]鲁东大学化学与材料科学学院,山东烟台264025

出　　处：《分析测试学报》2008年第10期1110-1113,共4页Journal of Instrumental Analysis

摘　　要：该文利用荧光光谱和吸收光谱研究了三磷酸腺苷二钠（ATP）与依诺沙星（ENX）和Tb^3＋的相互作用。依诺沙星与Tb^3＋形成二元络合物发射出位于545nm处的特征荧光，加入三磷酸腺苷二钠（ATP）后，体系的荧光强度显著增强，且增强的荧光强度与ATP的加入量成正比，据此建立了荧光分光光度法测量ATP的方法。实验测得，ATP的线性范围是2．00～20．0μmol·L^-1检出限为6．83×10^-8mol·L^-1。研究了共存物质的影响和Tb^3＋ -依诺沙星与三磷酸腺苷二钠的相互作用机理。该法成功地用于样品中ATP的测定。Based on the fact that the fluorescence intensity emitted by the enoxacin （ENA） - Tb^3＋ complex at the wavelength of 545 nm could be significantly enhanced in the presence of adenosine disodium triphosphate （ATP）, a fluorescence spectrometric method was developed for the determination of ATP. The effects of pH value, concentrations of ENX and Tb^3＋, reaction time, sequence of adding the reagents and coexisting substances on the fluorescence intensity were studied. Under optimum conditions, the linear range for ATP was 2.00 -20. 00 μmol·L^-1 with a detection limit of 6. 83×10^-8 mol·L^-1. The method has been applied to the determinations of ATP in various samples with satisfactory results. The mechanism of the interaction between enoxacin（ENX） -Tb^3＋ complex and ATP was also studied.

关 键 词：三磷酸腺苷二钠(ATP) 依诺沙星(ENX) TB^3+ 荧光光度法 

分 类 号：O657.31[理学—分析化学] Q524.6[理学—化学]