^(60)Coγ射线照射淋巴细胞诱导PIG3和GADD45基因mRNA变化  

Changes of PIG3 and GADD45 gene expression induced by ^(60)Coγ-rays in lymphoblastoid cells

作　　者：潘艳[1] 李玉文[1] 封江彬[1] 陆雪[1] 刘青杰[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《辐射研究与辐射工艺学报》2008年第5期309-313,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30570551);卫生部卫生行业科研专项(200802018)资助

摘　　要：用^(60)Coγ射线照射正常人外周血永生化淋巴细胞系后,初步分析受照细胞中PIG3和GADD45基因mRNA表达水平的改变状况,并探讨上述基因作为辐射生物剂量计的可能性及意义。用^(60)Coγ射线照射淋巴细胞,采用不同剂量(0～10Gy)照射和照射后不同时间点(0～72h)收集细胞,抽提mRNA,用Real-time PCR检测细胞中PIG3和GADD45基因mRNA表达水平的变化。结果表明,正常人外周血永生化淋巴细胞系中PIG3和GADD45基因mRNA表达水平随照射剂量的增高而增加,并呈现出剂量效应关系。5Gy剂量^(60)Coγ射线照射后,淋巴细胞中PIG3和GADD45基因mRNA表达水平随时间的推移不断增高,在8h时均达到最高,随后开始降低。PIG3和GADD45基因对辐射均较敏感,其表达具有良好的剂量效应和时间效应关系,但相比而言,PIG3的表达随照射剂量和照射后时间的变化更加显著,更有潜力作为生物剂量计以满足实际需要。This study is try to investigate the changes of PIG3 and GADD45 gene mRNA expression after irradiation and the probability of them as bio-dosimeters. In the dose-effect study, lymphoblastoid cells were irradiated by^60Coγ-rays with the doses of 0-10Gy and cultivated for 8 hours. Lymphoblastoid cells were cultivated for 0-72h after 5Gy irradiation for the time-effect study. The mRNA was extracted and real-time PCR method was used to detect the changes of PIG3 and GADD45 gene mRNA expression level in lymphoblastoid cells. The results show that PIG3 and GADD45 gene expression level in lymphocytes increases in a dose-dependent manner and has good dose-effect relationship. The time-effect curve indicates that the peak expression of PIG3 and GADD45 is at 8h after 5Gy irradiation and then begins to decrease gradually. PIG3 and GADD45 gene are sensitive to irradiation and both of them have dose-effect and time-effect relationships.

关 键 词：辐射损伤 淋巴细胞 PIG3 GADD45 基因表达 

分 类 号：R811.5[医药卫生—放射医学] Q691.5[医药卫生—临床医学]