用原生质体融合技术提高植物内生放线菌*的抗菌活性  

Improvement of endophytic actinomycetes by protoplast fusion

作　　者：陈丽萍[1] 宗兆锋[1] 李春玲[1] 郭萍萍[1] 骞天佑[1] 

机构地区：[1]西北农林科技大学植保学院与陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第10期158-162,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：教育部创新团队发展计划项目(200558);国家高等学校学科创新引智计划项目(B07049);西北农林科技大学创新团队项目

摘　　要：【目的】提高植物内生放线菌SF4和SG2的抗菌活性,获得具有更好生防效果的工程放线菌,为植物病害生物防治奠定基础。【方法】运用原生质体融合技术,将SF4、SG2和SC1进行融合,研究融合菌株的皿内抑菌活性及持续抑菌作用,并测定了其内生性和多胺效果。【结果】筛选获得了12株形态特征和菌落颜色与亲本菌株有差异、且遗传性状稳定的融合菌株,其中F-G1、F-G7、F-G11、F-G12、F-G15和G-C5等6株融合菌株对病原真菌有较强的抑制作用;菌株F-G11对灰葡萄孢(Botrytis cinerea)的抑制率为71.5%,F-G15对西瓜枯萎病菌(Fusarium oxys-porumf.sp.niveum)和梨状毛霉(Mucor piriformis)的抑制率达到73%,且有持续抑制作用;融合菌株F-G1、F-G11和F-G15继承了亲本的内生性和广泛定殖性;6株融合菌株均能产生多胺。【结论】原生质体融合技术实现了植物内生放线菌之间,以及植物内生放线菌和土壤中生防放线菌之间的融合,是改良放线菌特性的有效手段。【Objective】 The study was to improve the biocontrol character of endophytic actinomycetes SF4,SG2.【Method】 Technology of protoplast fusion was used to improve the wild strains.【Result】 Twelve recombinant strains were isolated based on the morphological character,color of colony and stable genetic determinant,six effective recombinant strains：F-G1,F-G7,F-G11,F-G12,F-G15 and G-C5,which have strongest inhibition to target pathogen,were screened.The strain F-G15 had obvious inhibiting effects（〉73%） and had permanent inhibiting effects on Fusarium oxysporum f.sp.niveum and Mucor piriformis,the strain F-G11 had same effects（〉70%） on Botrytis cinerea Recombinant strains F-G1,F-G7,F-G11,F-G12,F-G15 and G-C5were isolated from three different plants and also can produce polyamines.The parents＇ endophytic character,wide colonization and producing polyamines were inherited by six recombinant strains.【Conclusion】 Apparently,achieving protoplast fusion between endophytic antinomycetes and biocontrol antinomyectes by the technology of protoplast fusion is an effective method to improve the endophytic actinomycetes character.

关 键 词：生防放线菌SC1 植物内生放线菌 原生质体融合 西瓜枯萎病菌 抗菌活性 

分 类 号：Q939.132[生物学—微生物学]