丙酮醛诱导细胞凋亡相关基因SHMT2L的生物信息学分析  

Bioinformatics Analysis for Cell Apoptosis Induced by Methylglyoxal Related Gene SHMT2L

作　　者：曾军英[1] 刘学伟[2] 张杰[2] 孙志洪[1] 谭支良[3] 

机构地区：[1]中国科学院研究生院,中国北京100039 [2]云南大学,中国云南昆明650091 [3]中国科学院亚热带农业生态所,中国湖南长沙410125

出　　处：《生命科学研究》2008年第2期131-135,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30260027);国家自然科学基金资助项目(30571352)

摘　　要：基因芯片是一种高通量地同时研究多个基因表达的有力工具.在用基因芯片技术检测丙酮醛诱导人牙周膜成纤维细胞凋亡的基因表达研究中,发现一个新基因SHMT2L,其表达为显著下调,并推测该基因与细胞凋亡密切相关.利用生物信息学方法对SHMT2L基因及其蛋白产物进行各种分析,结果发现SHMT2L基因与线粒体丝氨酸羟甲基转移酶具有较高的相似性.SHMT2L在细胞中低表达与丙酮醛造成DNA损伤所引发的细胞凋亡相关.cDNA microarray technology is a powerful and efficient tool for large-scale screening differentially expressed genes simultaneously. Previous studies in our lab have shown a down-regulated gene expression （SHMT2L） which may be related to apoptosis of human periodontal ligament fibroblasts （HPDLFs） induced by methylglyoxal（MG）. Bioinformatics analytic methods were used to further understand the homology, structure of gene and potential functions and location for its coded protein. The results have shown SHMT2L has great homology with mitochondrial serine hydroxymethytransferase. The down expression of SHMT2L could lead to DNA damage including oxidative lesions, resulted in apoptosis of HPDLFs induced by MG.

关 键 词：丙酮醛 基因芯片 SHMT2L 生物信息学 

分 类 号：Q75[生物学—分子生物学]