肺炎支原体抗体检测方法比较及临床意义  

Comparison Between the Two Serological Test Methods for Detection of Mycoplasma Pneumoniae Antibody

作　　者：胡志刚[1] 唐建英[1] 周颖[1] 

机构地区：[1]无锡市人民医院检验科,214023

出　　处：《放射免疫学杂志》2008年第5期390-391,共2页Journal of Radioimmanology

摘　　要：目的:探讨肺炎支原体(MP)早期诊断的实验室检测方法及血清肺炎支原体抗体滴度在判断儿童肺炎支原体感染病情中的临床价值。方法:对586例疑似支原体感染的患儿同时进行明胶颗粒法、酶联免疫吸附法检测肺炎支原体,评估两种方法的应用价值。回顾性分析374例肺炎支原体感染患儿的临床资料。用双变量Spearman等级相关分析研究应用间接凝集试验法检测所得的血清MP抗体的滴度与MP感染患儿病情的关系。结果:明胶颗粒法和ELISA间接法在检测MP抗体中差异无显著性。374例MP感染患儿中,轻度MP感染175例(46.8%),中度感染104例(27.8%),重度感染95例(25.4%);MP抗体滴度1∶80有140例(37.4%),滴度1∶160有78例(20.9%),滴度1∶320有58例(15.5%),滴度1∶640有29例(7.8%)。滴度1∶1280有69例(18.4%),经统计学分析,血清MP抗体滴度与MP感染患儿的病情严重程度呈正相关(r=0.689,P<0.01)。结论:明胶颗粒法更能精确测定抗体滴度,敏感度高,操作简单,MP抗体滴度的测定有助于判断患儿的病情,为临床采取有效的治疗方案提供依据,可在临床推广使用。Objective To explore the difference of the results obtained by two serological tests for detection of mycoplasma pneumoniae antibody. Methods Serum mycoplasma pneumoniae antibody was detected with both polystyrene latex agglutination reaction （PLA） and ELISA in 576 pediatric patients with the tentative diagnosis of mycoplasma pneumonia. Also the serum titer of mycoplasma pneumoniae antibody （with PLA） and the clinical presentation of the severity of disease in 374 clinically diagnosed patients with mycoplasma pneumonia were retrospectively analyzed. Results Among the 576 patients, the discrepancy between the results obtained from PLA and ELISA was insignificant. In the retrospective analysis of the data of 374 patients, it was found that the titer of the antibody was positively correlated with the severity of the disease （r=0.689, P〈0.01）. Conclusion Results of PLA are expressed in titers （1：80-1：1280）, which is more quantitative and may reflect the severity of the disease process. This method can be rapidly and easily performed with prompt result （3h）, is of low cost and is worth popularization.

关 键 词：肺炎支原体 明胶颗粒法 ELISA间接法 

分 类 号：R725.631[医药卫生—儿科] R446.62[医药卫生—临床医学]