瘦素受体基因Gln223Arg多态性与温州地区高血压合并肥胖的相关性研究  

Study on the relationship between Gln223Arg variation in leptin receptor gene and hypertension complicated with obesity in Wenzhou population

作　　者：潘若望[1] 石南松[2] 张恩勇[1] 黄大道[1] 应俊[3] 张洪勤[3] 李佩珍[3] 

机构地区：[1]中国人民解放军第118医院外科 [2]中国人民解放军91765部队医院外科 [3]温州医学院生物学实验教学中心

出　　处：《解放军医学杂志》2008年第10期1192-1194,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的了解温州地区汉族人群瘦素受体Gln223Arg基因型分布;探讨该基因多态性与高血压合并肥胖的关系。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定无亲缘关系的321例温州地区汉族人群的瘦素受体基因Gln223Arg多态性(对照组111例,高血压病合并肥胖组106例,高血压病非肥胖组104例)。结果高血压病合并肥胖组瘦素受体基因Gln223Arg的GG、GA和AA 3种基因型的表达频率分别为0.604、0.377和0.019,G和A等位基因频率分别为0.769和0.231;高血压病非肥胖组3种基因型的表达频率分别为0.712、0.279和0.010,G和A等位基因频率分别为0.865和0.135;对照组3种基因型的表达频率分别为0.802、0.189和0.009,G和A等位基因频率分别为0.896和0.104;Gln223Arg位点多态性的分布频率在对照组和病例组间差异有统计学意义(χ2=6.310,P<0.05),且A等位基因频率在高血压合并肥胖患者中显著高于非肥胖组(χ2=6.532,P<0.05)。结论瘦素受体基因Gln223Arg多态性与温州地区汉族人群高血压合并肥胖相关。Objective To study the genotype distribution of Gln223Arg in Han population in Wenzhou area, and discuss its association with hypertension patients with obesity. Methods The genotypes of Gln223Arg variant in leptin receptor gene were determined by polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） assay in 321 unrelated subjects of Wenzhou Han population,including 111 normal subjects （the control group）, 106 cases of hypertension patients with obesity and 104 cases of hypertension patients without obesity （the case group）. Results The frequencies of GG, GA and AA genotypes in patients of hypertension with obesity were 0. 604, 0. 377 and 0. 019, respectively, 0. 712, 0. 279 and 0. 010 in patients without obesity; 0. 802, 0. 189 and 0. 009 in control group. The frequencies of G and A allele were 0. 769, 0. 231 respectively in patients of hypertension with obesity, 0. 865, 0. 135 in patients of hypertension without obesity, 0. 869, 0. 104 in control group. The genotype frequency of Gln223Arg variant in leptin receptor gene in the control group and the case group had a significant difference （χ^2=6. 310, P〈（0. 05）. The A allele frequencies also showed significant difference between the patients of hypertension with obesity and the patients of hypertension without obesity （χ^2= 6. 532, P〈0. 05） and was significantly higher in the patients of hypertension with obesity than those in patients of hypertension without obesity. Condusion These data indicated that Gln223Arg variation in LEPR gene was possibly associated with hypertension complicated with obesity in Han population in Wenzhou area.

关 键 词：瘦素 受体 多态性 限制性片段长度 高血压 肥胖 

分 类 号：R544.1[医药卫生—心血管疾病]