葡萄根系钙处理对叶片镉伤害的保护作用  

Calcium Protects Grape Leaves Against Cadmium Stress by Root Treatment

作　　者：张鑫荣[1] 杨洪强[1] 隋静[1] 乔海涛[1] 姜倩倩[1] 冉昆[1] 由淑贞[1] 张龙[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《园艺学报》2008年第10期1405-1410,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671452)

摘　　要：以巨峰葡萄扦插幼苗为试材,通过根部处理,研究氯化镉(CdCl2)处理下氯化钙(CaCl2)对其叶片的保护作用。结果表明:0.5mmol·L-1 CdCl2处理显著增加叶片H2O2和MDA(丙二醛)含量以及质膜透性,抑制叶片CAT(过氧化氢酶)活性,降低呼吸速率和质膜H+-ATPase、Ca2+-ATPase活性。0.5mmol·L-1 CaCl2处理显著抑制由CdCl2引起的叶片MDA和H2O2含量以及电解质渗漏率的升高,减轻CdCl2对叶片CAT活性、呼吸速率以及质膜H+-ATPase和Ca2+-ATPase活性的抑制。这些结果显示,根部应用0.5mmol·L-1的CaCl2能够缓解CdCl2对葡萄叶片造成的伤害。Protecting effects of CaCl2 on the leaves of grape against cadmium stress were studied by root treatment using cutting seedling of Kyoho grape. The results showed that CdCl2 could induce significantly accumulation of MDA and H2O2 and increase electrolytic leakage of PM （ plasma membrane）, also markedly inhibited the respiratory rate, CAT activity, H^ ＋-ATPase and Ca^2＋-ATPase activities in PM. The application of 0.5 mmol· L^-1 CaCl2 inhibited significantly the increases of MDA and H2O2 content and electrolytic leakage of PM caused by CdCl2 treatment, and decreased the inhibition of CAT activity, respiratory rate and the activity of H ^＋ -ATPase and Ca^2＋ -ATPase in PM under CdCl2 treatment. These indicated that 0.5 mmol· L^-1 CaCl2 can alleviate the stress caused by CdCl2 in grape leaves.

关 键 词：葡萄 重金属 镉 钙 保护酶 呼吸作用 

分 类 号：S663.1[农业科学—果树学]