香蕉果实贮藏冷害与NAD激酶活性变化的关系  

The Relationship Between Chilling Injury and NADK Activity in Postharvest Banana Fruits

作　　者：刘彩霞[1] 顾采琴[1] 朱冬雪[2] 

机构地区：[1]广州大学化学化工学院食品系,广州510006 [2]广州大学生命科学学院,广州510006

出　　处：《园艺学报》2008年第10期1425-1430,共6页Acta Horticulturae Sinica

基　　金：广州大学人才引进基金资助项目(1001)

摘　　要：以‘巴西’香蕉果实为试材,研究了果实采后在22℃和6℃下贮藏期间NAD激酶(NADK)活性、冷害发生情况、膜渗透性、丙二醛(MDA)含量、多酚氧化酶(PPO)以及苯丙氨酸解氨酶(PAL)活性变化。结果表明,6℃下贮藏3d时果实出现冷害症状,11d时冷害加重,同时NAD激酶活性、膜渗透性、MDA含量和PPO活性均始终高于22℃贮藏的,并在11d时达到高峰,而PAL活性则呈下降趋势,其活性始终低于22℃贮藏的。6℃下用NAD激酶激活剂氯化钙处理香蕉果实后,NADK受到激活,冷害程度加重,而用钙离子通道阻塞剂异博定(verapamil,Vp)处理后则抑制了NADK活性,果实冷害症状延迟4d出现,冷害程度减弱,说明香蕉果实冷害发生与NADK活性变化密切相关。Changes of activity of NAD kinase, polyphenol oxidase （PPO） and phenylalanine ammonialyase （PAL）, chilling injury and relative conductivity, MDA content of postharvest banana fruits storage at 22 and 6 ℃ were studied. The results showed that the fruits at 6 ℃ for three days occured visible chilling injury and became more serious for 11 days. The activity of NAD kinase at 6 ℃ was higher than that at 22 ℃ during storage. Higher relative conductivity, MDA content and PPO activity were observed at 6 ℃ than those at 22 ℃ and reached peak on the 11 th day. However, the PAL activity at 6℃ was lower than that at 22℃ during storage. Pretreatment with CaCl2 could enhance the activity of NAD kinase and increase chilling injury of banana fruits at 6 ℃, but pretreatment with verapamil （Vp） had reverse effect. The results suggested that the chilling injury of postharvest banana fruits was highly related to the change of NADK activity.

关 键 词：香蕉 果实 冷害 NAD激酶 贮藏 

分 类 号：S668.1[农业科学—果树学]