用AFLP标记分析广西龙眼种质遗传多样性  

Genetic Diversity of Longan in Guangxi Assessed by AFLP Markers

作　　者：彭宏祥[1] 李冬波[1] 朱建华[1] 李江舟[2] 曹辉庆[1] 潘丽梅[2] 

机构地区：[1]广西农业科学院园艺研究所,南宁530007 [2]广西大学农学院,南宁530005

出　　处：《园艺学报》2008年第10期1511-1516,共6页Acta Horticulturae Sinica

基　　金：广西自然科学基金项目(桂科自0339023)

摘　　要：选用10对AFLP引物组合分析了广西38个龙眼品种(优良单株)和两个龙眼近缘亚种龙荔单株,以及10个来自国内外其他产区龙眼品种的遗传多样性。在所扩增的570条AFLP主带中,有485条具有多态性,占85.1%。所用引物可将供试的品种(优良单株)区分开来,供试材料的遗传相似系数在0.39(两个亚种间)至0.98之间。根据遗传相似系数(UPGMA,N-J)得到的分类树状图与传统方法的分类结果相类似。研究结果表明广西龙眼种质具有较广泛的遗传多样性。To determine the genetic diversity of longan （Dimocarpus longan Lour. ） varieties in Guangxi, we employed 10 pairs of AFLP primer combinations on 38 varieties and ：2 subspecific varieties [Dimocarpus confinis （How et Ho） H. S. Do] in Guangxi, and 10 varieties in other regions and countries. A total of 570 major AFLP bands were observed, and 485 bands （85.1% ） were polymorphism. All of the cultivars could be divided with the markers used. Genetic similarities among all taxa ranged from 0.39 （ between the two subspecies） to 0. 98. Phenetic trees based on genetic similarities （UPGMA, N-J） were similar to known taxonomic relationships. The results showed that the genetic diversity of longan is very high in Guangxi.

关 键 词：龙眼 遗传多样性 AFLP 

分 类 号：S667.2[农业科学—果树学]