益气活血法对大鼠萎缩性胃炎癌前病变差异基因表达谱的影响  

Effect of Qi-replenishing and blood-activating treatment on aberrant gene expression profile in the precancerous lesion of CAG in rats

作　　者：李军祥[1] 张玉禄[1] 朱陵群[2] 赵宁宁[1] 

机构地区：[1]北京中医药大学东方医院消化科,北京市100078 [2]北京中医药大学中医内科学教育部重点实验室

出　　处：《世界华人消化杂志》2008年第29期3261-3268,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30572388~~

摘　　要：目的:分析益气活血法干预慢性萎缩性胃炎(chronic atrophic gastritis,CAG)癌前病变的差异表达基因,从基因水平探讨益气活血法防治大鼠CAG癌前病变的药理机制方法:应用大鼠12K基因表达谱cDNA芯片检测益气活血法干预后CAG癌前病变大鼠基因的差异表达变化,筛选出差异表达基因建立益气活血法干预后CAG癌前病变大鼠差异基因表达谱并进行生物信息学分析.并运用Real-time荧光定量PCR鉴定HSP70基因表达.结果:共筛选出差异表达基因205个,其中上调基因为101条,下调基因104条,共同构成了益气活血法干预大鼠CAG癌前病变的差异表达基因谱.与凋亡相关的4个基因为heat shock 70kDa protein 1a,growth arrest and DNA-damage-inducible,complement component 9和albumin.Real-time荧光定量PCR鉴定结果表明益气活血法干预治疗后HSP70基因表达上调,与芯片检测结果一致.结论:益气活血法对大鼠CAG癌前病变的基因表达的影响涉及到多种生物学过程信号传递及基因调控的改变,从分子生物学角度说明中医益气活法对CAG癌前病变的影响是一个复杂的病理生理过程.AIM： To investigate aberrant gene expression of chronic atrophic gastritis （CAG） precancerous lesions in rats receoiving Qi-replenishing and blood-activating treatment and to analyze its pharmaco-mechanism at genetic level. METHODS： The model of precancerous lesion of CAG rats induced by spring insertion and hot paste was established. One step methodfor extraction of total RNA of rat stomach tissues after administration of Qi-replenishing and blood-activating medicinals and the stomach tissue of the model group was used. After reverse transcription and fluorescent labeling by Cy3, Cy5, we got two groups of rat stomach cDNA probes and then hybridized with the cDNA gene expression profiles microarray hybridization. The result was scanned by the laser scanner and processed by software for image analysis, standardization, the ratio of value analysis, cluster analysis and gene ontology analysis. Results of gene array were verified by Real-time PCR. RESULTS： Screening showed 205 differentially expressed genes, of which, there were 101 up-regulated expression genes and 104 down-regulated expression genes. They composed the gene expression profiles for precancerous lesion of precancerous lesions of CAG in rats treated with Qi-replenishing and blood-activating medicinals. There were four genes related to apoptosis including heat shock 70 kDa protein la, growth arrest and dna-damage-inducible, complement component 9 and albumin. The Real-time PCR confirmed expression of HSP70 up-regulated in rats treated with Qi-replenishing and bloodactivating medicinals, which were consistent with results using microarray technology. CONCLUSION： These differentially expressed genes involved different biological processes and gene regulations. Further analysis of the differentially expressed genes might help reveal the pharmaco-mechanism of Qi-replenishing and blood-activating medicinals at genetic level.

关 键 词：基因表达 基因芯片 萎缩性胃炎 癌前病变 大鼠 益气活血法 中医 

分 类 号：R259[医药卫生—中西医结合]