模拟失重对大鼠肠黏膜NF-κB表达的影响  

Effects of simulated gravity loss on nuclear factor kappa-B expression in rat intestinal tissues

作　　者：李成林[1,2] 张铭[2] 周金莲[2] 崔彦[2] 余佩武[1] 

机构地区：[1]中国人民解放军第三军医大学西南医院普通外科,重庆市400038 [2]中国人民解放军第306医院,北京市100101

出　　处：《世界华人消化杂志》2008年第29期3328-3331,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨模拟失重条件下健康大鼠肠道黏膜组织中核因子-κB(NF-κB)的表达变化及其意义.方法:成年Wistar大鼠80只,随机分为10组,按模拟失重时相分别为0.5、1、2、7、21d和相应的对照组.采用尾悬吊法建立模拟失重动物模型.免疫组织化学法检测肠道黏膜组织中NF-κB的表达水平.结果:0.5、1、2、7、21d模拟失重组大鼠肠道组织NF-κB表达水平均显著高于相应对照组(10.11%±3.29%vs5.50%±1.92%,22.00%±5.31%vs6.50%±2.32%,25.50%±4.11%vs8.75%±6.36%,21.50%±3.02%vs6.75%±2.12%,10.87%±2.64%vs5.62%±2.13%,均P<0.01),模拟失重0.5d组肠道组织NF-κB表达开始升高,2d组达高峰,随模拟失重时间的延长,NF-κB表达水平依次下降,21d组仍然高于相应对照组.结论:尾悬吊模拟失重大鼠肠道组织NF-κB表达水平明显上调,提示肠道组织NF-κB表达变化与失重应激反应和失重耐受有密切关系.达水平明显上调,提示肠道组织NF-κB表达变化与失重应激反应和失重耐受有密切关系.AIM： To investigate the effects of simulated gravity loss on NF-κB expression in rat intestinal tissues, and its significance. METHODS： Tail-suspension （TS） was used to simulate the physiological effects of gravity loss. Eighty male Wistar rats were randomly assigned to 10 experimental groups suspended for 0.5, 1, 2, 7, 21 d respectively and their corresponding control groups. The dynamic expressions of NF-κB in intestinal tissues were seperately measured using the immunohistochemistry. RESULTS： Compared with control groups, the expression levels of NF-κB in the intestinal tissue of tail-suspension 0.5, 1, 2, 7, 21 d groups were elevated significantly （10.11% ± 3.29% vs 5.50% ± 1.92%, 22.00% ± 5.31% vs 6.50%± 2.32%, 25.50% ± 4.11% vs 8.75% ± 6.36%, 21.50%± 3.02% vs 6.75% ± 2.12%, 10.87% ± 2.64% vs 5.62% ± 2.13%, all P 〈 0.01）. NF-κB expression level began to rise in 0.5 tail-suspension group, and reached the maxium level in in 2 d group, then went down gradually. CONCLUSION： The results suggest that simulated gravity loss acts as a kind of stress to elevate NF-κB expression and indicate a close link between altered intestinal NF-κB expression and tolerance of gravity loss.

关 键 词：核因子-ΚB 模拟失重 大鼠 肠黏膜 免疫组织化学法 

分 类 号：R85[医药卫生—航空、航天与航海医学]