转GFM CrylA基因玉米自交系及其杂交种的抗螟性鉴定  

Identifications of Resistance to Corn-borer of Transgenic Maize Inbred Lines and Hybrids with GFM CrylA Gene

作　　者：袁英[1] 李晓辉[1] 孔祥梅[1] 姜志磊[1] 林春晶[1] 李葱葱[1] 刘娜[1] 孙传波[1] 刘德璞[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130124

出　　处：《农业生物技术学报》2008年第5期831-836,共6页Journal of Agricultural Biotechnology

基　　金：国家转基因中试及产业化基地专项(No.JY03-17-1)资助

摘　　要：对获得的6个转GFM CrylA基因玉米(Zea mays)株系的T5代PCR分析证明,转基因株系是稳定遗传的。以此抗虫系为亲本与常规自交系配制了四密25Bt、通单24Bt和吉单209Bt3个杂交种。转基因自交系和杂交种的抗螟性鉴定结果表明:转基因系间抗虫性差异极显著,同一转基因系单株间也存在差异。抗螟性强的4个株系比受体对照平均蛀孔数减少70%、茎秆虫孔隧道长度减少80%、存活的虫体长度减少70%,差异显著,达到了高抗水平。对6个转基因抗虫系进ELISA检测,毒蛋白表达量平均占总蛋白0.16%,最高的达0.19%。杂交组合四密25Bt、通单24Bt和吉单209Bt群体内平均虫孔隧道长度与常规杂交种对照相比,分别减少39.97%、36.2%和53.83%,平均减少43.36%,抗虫性明显提高。农艺性状鉴定结果显示,转基因系配制的杂交种与对照在株高、穗长、穗行数、行粒数和百粒重上无显著差异。研究认为,Bt基因可用于玉米的抗螟性改良,为转基因抗虫玉米自交系能够直接用于玉米杂交种改良提供了依据。Six transgenic lines with GFM CrylA had been obtained and PCR analysis of their T5 generation plants indicated that foreign genes could be stable inheritance. Three hybrids （Simi25^Bt, Tongdan24^Bt and Jidan209^Bt were created using the transgenic lines combined with regular maize inbred lines. The results of resistance identification of the transgenic inbred lines and hybrids showed that the difference of resistance among transgenic lines was very significant; there also existed difference among individuals of the same line and among the three hybrids. Compared with the control inbred line （CK）, the average of 4 transgenic inbred lines in the tunnels per stalk, larvae tunnel length per plant and survived larval length were decreased by 70%, 80% and 70%,respectively. ELISA testing showed that the average of Bt Cry l A protein expression level for 6 transgenic inbred lines was 0.16% of total protein and the highest expression level was 0.19%. Comparing with the control variety （CK）, the larvae tunnel length per plant of 3 hybrids （Simi25^Bt, Tongdan24^Bt and Jidan209a） was decreased by 39.97%, 36.20% and 53.83%, respectively, which was decreased 43.36% on average. The investigation of agronomic characters showed that there was no significant difference between improved hybrids and CK in plant height, ear length, row number per ear, kernel number per row and 100-kernels weight. It is thought that the GFM CrylA gene can be applied to the improvement of resistance to corn-borer, and maize inbred lines with Bt gene can be directly applied to conventional maize breeding.

关 键 词：玉米 GFM CrylA基因 自交系 杂交种 

分 类 号：S188[农业科学—农业基础科学]