玫瑰黄链霉菌Men-myco-93-63及其抗生素高产菌株差异蛋白分析  

Analysis of Differential Protein between Streptomyces roseoflavus Men-myco-93-63 and Its High-yield Antibiotic Strain

作　　者：李爱霞[1] 李亚宁[1] 张娜[1] 闫红飞[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《农业生物技术学报》2008年第5期886-890,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30800734);国家高技术研究与发展计划(863)(No.2006AA10A211)资助

摘　　要：玫瑰黄链霉菌(Streptomyces roseoflavus)Men-myco-93-63是分离自马铃薯疮痂病自然衰退土壤中的一株拮抗菌。该菌株及其发酵液对棉花黄萎病菌(Verticillium dahliae)和瓜类白粉病菌(Sphaerotheca fuliginea Poll)等多种重要的植物病原真菌表现出较强的抑制作用,具有良好的生防应用潜力。利用蛋白质双向凝胶电泳技术研究了Men-myco-93-63与其抗生素高产菌株D12-3之间的蛋白质表达差异。通过优化条件,建立了一套适合该生防菌的双向电泳体系。通过比较蛋白质图谱的差异,发现了4个差异蛋白。质谱分析表明,这4个蛋白点分别为聚羟基脂肪酸(PHAs)的包涵体蛋白(PhaP)、β-内酰胺酶(Beta-lac-tamase)、甲基接受趋化蛋白(methyl-accepting chemotaxis protein)和ABC转运体(ABC transporter,ATP-binding protein),这些蛋白可能与该生防菌的拮抗活性有关。Streptomyces roseoflavus Men-myco-93-63 isolated from potato scab （S. scabies） decline soil and its fermentation can inhibit many phytopathogenic fungi and control related important plant diseases such as cotton verticillium wilt （Verticillium dahliae） and cumber powdery mildew （Sphaerotheca fuliginea Poll）. The total protein of Streptomyces roseoflavus Men-myco-93-63 and its high-yield antibiotic strain D12-3 were analyzed by two-dimensional gel electrophoresis. As the results, a 2-DE protocol and electrophoresis program for Men-myco-93-63 were developed. Four different expression protein spots were isolated and identified by matrix-assisted laser desorption ionization （MALDI）-time-of-flight （TOF） -mass spectrum （MS）. The results showed that these proteins were PhaP, Beta-lactamase, methyl-accepting chemotaxis protein and ABC transporter, respectively, which perhaps are related with the biosynthesis of antibiotics of S. roseoflavus Men-myco-93-63.

关 键 词：玫瑰黄链霉菌 抗生素高产菌株 二维凝胶电泳 质谱分析 

分 类 号：S188[农业科学—农业基础科学]