He-Ne激光对UV-B辐射小麦幼苗糖代谢的影响  

Effects of He-Ne Laser on Sugar Metabolism of Wheat Seedling Exposed to Ultraviolet-B Radiation

作　　者：张娟[1] 王小花[1] 郝金花[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《激光生物学报》2008年第5期577-581,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30671061);山西省自然科学基金项目(20041101)

摘　　要：分别采用5mW·mm-2He-Ne激光辐照、10.08kJ·m-2.d-1增强UV-B辐射及二者组合对"晋麦8号"小麦幼苗进行处理,测定各处理幼苗叶片可溶性糖、还原性糖、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)以及α,β-淀粉酶的变化。研究分析He-Ne激光对增强UV-B辐射引起小麦损伤的修复效应。结果表明:He-Ne激光辐照可使UV-B辐射后小麦幼苗可溶性糖含量升高,还原性糖含量降低,SS,SPS活力降低,α,β-淀粉酶活力升高。该结果同时表明可溶性糖、还原性糖、SS、SPS、α,β-淀粉酶的变化同小麦幼苗损伤修复的能力相关,一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗糖代谢的辐射损伤。The seedling of wheat （ Triticum aestivum） were exposed to He-Ne laser irradiation with 5 mW · mm^-2 power density, enhanced UV-B radiation （ 10.08 kJ ·m^-2· d^-1 ） and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation. Changes on the soluble sugar, reducing sugar, sucrose synthase （SS） , sucrose phosphate synthase （SPS） and α, β-amylase were measured to test the repairing role of He-Ne laser irradiation. The results showed that He-Ne laser irradiation on the wheat seedlings resulted in increase of the content soluble sugar and decrease of the content of reducing sugar, the activity of SS, SPS decreased and α, βamylase enhanced. It suggested that those changes in production rate of soluble sugar, reducing sugar, SS, SPS and α, β-amylase were responsible for the capacity of repairing in the wheat. Therefore, the damage of wheat seedlings induced by enhanced UV-B radiation on sugar metabolism can be repaired partly by He-Ne laser irradiation.

关 键 词：He—Ne激光 UV—B辐射 小麦 糖代谢 修复效应 

分 类 号：Q493.4[生物学—生理学] S512.1[农业科学—作物学]