正交试验优选复方百部止咳颗粒中黄芩苷的提取工艺  

Optimized Procedures for Baicailin Extraction from Fufang Baibu Zhike Granules Based on Orthogonal Design

作　　者：戴航[1] 侯小涛[1] 程世贤[1] 

机构地区：[1]广西中医学院,广西南宁530001

出　　处：《世界中西医结合杂志》2008年第3期139-141,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：广西高校人才小高地建设创新团队基金资助项目

摘　　要：目的:研究复方百部止咳颗粒中黄芩苷的提取工艺。方法:以甲醇、乙醇为溶剂,选择最佳提取溶剂,从各种传统提取分离方法中,选择最佳提取方式,通过正交试验优选复方百部止咳颗粒中黄芩苷的提取工艺。结果:以乙醇做溶剂所得含量较高,且分离度较好,超声提取法所得黄芩苷含量较高,溶剂浸提法和回流提取法提取时间较长,超声提取法提取时间短,且无其他组分峰干扰,以80%乙醇作为提取溶媒所得到的黄芩苷含量最高,使用6倍量乙醇作为提取溶媒比使用8倍量、10倍量乙醇所得黄芩苷的含量高,超声时间超过40min后所得黄芩苷的含量已基本稳定。结论:复方百部止咳颗粒中黄芩苷的最佳提取工艺为6倍量80%乙醇,超声40min。Objective：To optimize the procedures for extracting baicailin from Fufang Baibu Zhike granules （FFBBZKG）. Methods：FFBBZKG was ultrasonically extracted and determined by HPLC with methyl alcohol and ethanol as solution. Baicailin extraction procedures were optimized by orthogonal design. Results：Baieailin could be obtained in a shorter time with more content in ethanol solution and by ultrasound. Most baicailin can be extracted in 80% ethanol solution. More baicailin was obtained in the solution with 6 times of ethanol than with 8 or 10 times of ethanol. The content of baicailin was stable with ultrasound after 40 min. Conclusion：The optimal condition for baicailin extraction was 6-time volume of 80% ethanol by 40min ultrasound.

关 键 词：正交试验 复方百部止咳颗粒 黄芩苷 

分 类 号：R284.2[医药卫生—中药学]