骨碎补总黄酮对急性肾衰竭大鼠ICAM-1基因表达的影响  

Effect of Assemble Flavones of Fortune's Drynaria Rhizome on the Expression of ICAM-1 in Rats with Acute Renal Failure

作　　者：蒋文功[1] 方敬爱[2] 孟民杰[1] 罗颖[1] 刘思敏[1] 陈燕[1] 周嫦容[1] 

机构地区：[1]广东药学院临床医学院,广州510006 [2]山西医科大学第一医院肾内科,太原030001

出　　处：《中国中西医结合肾病杂志》2008年第10期856-859,I0001,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广东省医学科研基金立项项目(No.A2007303)

摘　　要：目的:研究骨碎补总黄酮(AFFDR)对大肠杆菌脂多糖(LPS)诱导的急性肾衰竭大鼠肾组织细胞间黏附因子-1(ICAM-1)mRNA表达的影响。方法:用LPS(30mg/kg)腹腔注射建立急性肾衰竭大鼠模型,分模型组(LPS组)、骨碎补总黄酮治疗组(LPS+AFFDR组)、正常对照组(Control组)和骨碎补对照组(AFFDR组)。实验第7天测定各组大鼠血清肌酐(Scr)的含量,观察肾脏的超微结构和ED-1阳性巨噬细胞的浸润,并用半定量RT-PCR方法检测AFFDR对肾组织ICAM-1 mRNA表达的影响。结果:LPS引起大鼠Scr明显升高,肾脏近曲小管出现明显病理改变。AFFDR有效降低LPS攻击大鼠Scr的含量,明显减轻近曲小管的损伤。LPS组肾组织ED-1阳性巨噬细胞浸润和ICAM-1 mRNA的表达明显高于对照组,而LPS+AFFDR组肾组织ED-1阳性巨噬细胞的浸润和ICAM-1 mRNA的表达明显低于LPS组。结论:AFFDR防治内毒性急性肾衰竭的作用机制可能与其抑制肾组织ED-1阳性巨噬细胞浸润和ICAM-1的表达有关。Objective： To investigate the effect of Assemble Flavones of Fortune＇s Drynaria Rhizome（AFFDR） on the mR- NA expression of renal intercellular adhesion molecule - 1 （ ICAM - 1 ） in rats with acute renal failure induced by lipopolysaecharide （LPS）. Methods：Acute renal failure rats model were established by intraperitoneal injection with LPS（30 mg/kg）,and then divided into LPS group,AFFDR treatment group （LPS ＋ AFFDR group） .normal control group and AFFDR group. On the 7th day after experiment, blood was collected for determining creatinine （Scr） concentrations, renal tissue for examining the expression of ED- 1 positive maerophage. In addition, electron microscopy was used to examine the ultrastructure changes in kidney, and RT- PCR was performed to detect renal mRNA expression of intercellular adhesion molecule - 1 （ICAM - 1 ）. Results： LPS significantly increased Ser concentrations, and produced an obvious pathological change in renal ultrastructure, which was significantly attenuated by AFFDR treatment. Moreover,AFFDR treatment markedly suppressed an increase in renal ICAM- 1 mRNA expression induced by LPS. Conclusion：These results suggest that AFFDR protects against LPS - induced acute renal failure through inhibiting ED- 1 positive macrophage infiltration and ICAM- 1mRNA expression in renal tissue.

关 键 词：骨碎补 急性肾衰竭 细胞间黏附因子 脂多糖 

分 类 号：R285.5[医药卫生—中药学]